Recombinant
RabMAb

Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Overview

  • Product name
    Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker
    See all Fibrillarin primary antibodies
  • Description
    Rabbit monoclonal [EPR10822(B)] to Fibrillarin - Nucleolar Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Fibrillarin. The exact sequence is proprietary.

  • Positive control
    • HeLa, HepG2, 293T and Molt4 cell lysates; Human colonic carcinoma and prostate tissues; HepG2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
    • Sequence similarities
      Belongs to the methyltransferase superfamily. Fibrillarin family.
    • Post-translational
      modifications
      By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
    • Cellular localization
      Nucleus, nucleolus. Fibrillar region of the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 34 kD nucleolar scleroderma antigen antibody
      • 34 kDa nucleolar scleroderma antigen antibody
      • Fbl antibody
      • FBRL_HUMAN antibody
      • FIB antibody
      • FIB1 antibody
      • FLRN antibody
      • Histone-glutamine methyltransferase antibody
      • Nop1p antibody
      • RNA U3 small nucleolar interacting protein 1 antibody
      • RNU3IP1 antibody
      • rRNA 2' O methyltransferase fibrillarin antibody
      • rRNA 2'-O-methyltransferase fibrillarin antibody
      see all

    Images

    • All lanes : Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : HepG2 lysate
      Lane 3 : 293T lysate
      Lane 4 : Molt4 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 33 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling Fibrillarin with ab154806 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Fibrillarin with ab154806 at 1/250 dilution.
    • Immunofluorescence analysis of HepG2 cells labeling Fibrillarin with ab154806 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154806 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154806 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154806 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154806 showing +ve staining.

    References

    ab154806 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HES2 human embryonic stem cells, 293t, HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    HES2 human embryonic stem cells, 293t, HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Jianlong Wang

    Verified customer

    Submitted Dec 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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