Recombinant
RabMAb

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Overview

  • Product name
    Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker
    See all Fibrillarin primary antibodies
  • Description
    Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Fibrillarin.

  • Positive control
    • 293T, HeLa, HepG2 and MOLT4 cell lysates; Human liver and Human testis tissues; HeLa cells. Permeabilized MOLT4 cells; Immunoprecipitation pellet from HepG2 cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/5000.

For unpurified use at 1/100 - 1/250

IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
  • Sequence similarities
    Belongs to the methyltransferase superfamily. Fibrillarin family.
  • Post-translational
    modifications
    By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
  • Cellular localization
    Nucleus, nucleolus. Fibrillar region of the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 34 kD nucleolar scleroderma antigen antibody
    • 34 kDa nucleolar scleroderma antigen antibody
    • Fbl antibody
    • FBRL_HUMAN antibody
    • FIB antibody
    • FIB1 antibody
    • FLRN antibody
    • Histone-glutamine methyltransferase antibody
    • Nop1p antibody
    • RNA U3 small nucleolar interacting protein 1 antibody
    • RNU3IP1 antibody
    • rRNA 2' O methyltransferase fibrillarin antibody
    • rRNA 2'-O-methyltransferase fibrillarin antibody
    see all

Images

  • All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MOLT4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 33 kDa

  • Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Fibrillarin (green) with purified ab166630 at 1/5000. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.  

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • ab166630 showing +ve staining in Human gastric adenocarcinoma.
  • ab166630 showing +ve staining in Human normal tonsil.
  • ab166630 showing +ve staining in Human melanoma.
  • ab166630 showing +ve staining in Human glioma.
  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Fibrillarin with ab166630 at a 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Fibrillarin with ab166630 at a 1/100 dilution.
  • Immunofluorescent analysis of HeLa cells labeling Fibrillarin with ab166630 at a 1/100 dilution.
  • Flow cytometric analysis of permeabilized MOLT4 cells using ab166630 at a 1/10 dilution (red) or a Rabbit IgG (negative) (green).
  • Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate at 10 µg

    Predicted band size: 33 kDa

References

ab166630 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HES2 human embryonic stem cells, 293T, HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
HES2 human embryonic stem cells, 293T, HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Jianlong Wang

Verified customer

Submitted Dec 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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