Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)


  • Product name
    Anti-Fibrinogen alpha chain antibody [EPR2919]
    See all Fibrinogen alpha chain primary antibodies
  • Description
    Rabbit monoclonal [EPR2919] to Fibrinogen alpha chain
  • Host species
  • Specificity
    The immunogen is derived from isoform alpha-E, UniProt accession P02671-1, and the antibody is not expected to detect isoform alpha.
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    within Human Fibrinogen alpha chain aa 750-850. The exact sequence is proprietary. The immunogen is derived from isoform alpha-E, UniProt accession P02671-1, and the antibody is not expected to detect isoform alpha.
    Database link: P02671-1

  • Positive control
    • Human hepatocellular carcinoma tissue Human plasma, fetal liver and HepG2 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab92572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP 1/50.
IHC-P 1/50 - 1/100.

Antigen retrieval plus the use of an HRP/AP polymerized secondary antibody is highly recommended for enhanced staining.

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • Tissue specificity
    • Involvement in disease
      Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
      Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • Sequence similarities
      Contains 1 fibrinogen C-terminal domain.
    • Domain
      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • Post-translational
      The alpha chain is not glycosylated.
      Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
      About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Ac1873 antibody
      • Fba5e antibody
      • FGA antibody
      • Fib antibody
      • Fib2 antibody
      • FIBA_HUMAN antibody
      • Fibrinogen alpha chain antibody
      • Fibrinogen, A alpha polypeptide antibody
      • Fibrinogen--alpha polypeptide chain antibody
      • Fibrinopeptide A antibody
      see all


    • All lanes : Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572) at 1/2000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 95 kDa

    • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab92572 at 1/2000 dilution.


    This product has been referenced in:
    • Rancourt RC  et al. Antifibrinolytic mechanisms in acute airway injury after sulfur mustard analog inhalation. Am J Respir Cell Mol Biol 51:559-67 (2014). Read more (PubMed: 24796565) »

    See 1 Publication for this product

    Customer reviews and Q&As

    The immunogen sequence used to generate ab92572 is a short peptide found with Fibrinogen alpha chain. It does not specifically detect the Fibrinopeptide A molecule.

    Thank you for your enquiry. The immunogen contains Fibrinogen alpha chain and is in the Fibrinogen C-terminal region. This antibody detects the main protein Fibrinogen. If Fibrinopeptide A has not been cleaved from Fibrinogen alpha chain, then ...

    Read More


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