Overview

  • Product name
  • Description
    Rabbit polyclonal to Fibrinogen
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, Immunodiffusionmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    Full length native protein (purified) corresponding to Pig Fibrinogen. (Isolated from fresh plasma after removing prothrombin).
    Database link: P14460

  • Positive control
    • Pig plasma.

Properties

  • Form
    Lyophilised:Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Diluted stock solutions should be used immediately and not be frozen.
  • Concentration information loading...
  • Purity
    Whole antiserum
  • Purification notes
    Delipidated, heat inactivated, stable whole antiserum. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.

Precipitin titre 1:64 when tested against pooled normal pig plasma in agar-block immunodiffusion titration.

Target

  • Function
    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications
    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGA antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen A alpha polypeptide antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen B alpha polypeptide antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen G alpha polypeptide antibody
    • Fibrinogen gamma chain antibody
    • fibrinogen, B beta polypeptide antibody
    • fibrinogen, G gamma polypeptide antibody
    • fibrinogen, gamma polypeptide antibody
    • Fibrinogen--alpha -polypeptide chain antibody
    • Fibrinogen--beta -polypeptide chain antibody
    • Fibrinogen--gamma-polypeptide chain antibody
    see all

References

ab183109 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ELISA
Sample
Pig Serum (Protein adsorbed on a solid surface)
Specification
Protein adsorbed on a solid surface
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type
Indirect
Username

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Verified customer

Submitted Jul 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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