Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)

Overview

  • Product name
    Anti-Fibrinogen gamma chain antibody [5A6]
    See all Fibrinogen gamma chain primary antibodies
  • Description
    Mouse monoclonal [5A6] to Fibrinogen gamma chain
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 210-437 of Human Fibrinogen gamma chain

  • Positive control
    • Recombinant Fibrinogen gamma chain protein, NIH3T3 cells
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR240839-12 are from Tissue Culture Supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    5A6
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in disease
    Defects in FGG are a cause of thrombophilia.
    Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Sulfation of C-terminal tyrosines increases affinity for thrombin.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGG antibody
    • FIBG_HUMAN antibody
    • Fibrinogen gamma chain antibody
    • Fibrinogen gamma polypeptide antibody
    • fibrinogen gamma-b chain antibody
    see all

Images

  • Anti-Fibrinogen gamma chain antibody [5A6] (ab119948) at 1/500 dilution + Recombinant Fibrinogen gamma chain protein

    Predicted band size : 52 kDa
  • Immunofluorescence analysis of NIH3T3 cells using ab119948 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA, using ab119948 at a dilution of 1/10000.

References

This product has been referenced in:
  • Wu L  et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). Read more (PubMed: 28534980) »
  • Benito-Jardón M  et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. Elife 6:N/A (2017). Read more (PubMed: 28092265) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20%)
Blocking step
Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

Submitted Dec 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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