Anti-Fibrinogen gamma chain antibody (ab62527)


  • Product nameAnti-Fibrinogen gamma chain antibody
    See all Fibrinogen gamma chain primary antibodies
  • Description
    Rabbit polyclonal to Fibrinogen gamma chain
  • Specificityab62527 will bind to the fibrin gamma chain as well as the fibrinogen gamma chain.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 289-338 of Human Fibrinogen gamma chain (AMFKVGPEAD KYRLTYAYFA GGDAGDAFDG FDFGDDPSDK FFTSHNGMQF)

  • Positive control
    • Fetal liver cell lysate.



Our Abpromise guarantee covers the use of ab62527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in diseaseDefects in FGG are a cause of thrombophilia.
    Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Sulfation of C-terminal tyrosines increases affinity for thrombin.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGG antibody
    • FIBG_HUMAN antibody
    • Fibrinogen gamma chain antibody
    • Fibrinogen gamma polypeptide antibody
    • fibrinogen gamma-b chain antibody
    see all

Anti-Fibrinogen gamma chain antibody images

  • Anti-Fibrinogen gamma chain antibody (ab62527) at 2.5 µg/ml + Fetal liver cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References for Anti-Fibrinogen gamma chain antibody (ab62527)

ab62527 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Spleen)
Loading amount 10 µg
Specification Spleen
Treatment FasL for 5 hours
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

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Submitted May 23 2012