Recombinant
RabMAb

Anti-Fibrinogen gamma chain antibody [EPR3084] (ab92481)

Overview

  • Product name
    Anti-Fibrinogen gamma chain antibody [EPR3084]
    See all Fibrinogen gamma chain primary antibodies
  • Description
    Rabbit monoclonal [EPR3084] to Fibrinogen gamma chain
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Fibrinogen gamma chain (UniProt ID: P02679).

  • Positive control
    • WB: Human plasma lysate IHC-P: Human liver tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/250. Heat mediated antigen retrieval is recommended before commencing with IHC staining protocol. HRP/AP polymerized antibody is recommended for use as a secondary antibody.
    WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • Involvement in disease
      Defects in FGG are a cause of thrombophilia.
      Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
    • Sequence similarities
      Contains 1 fibrinogen C-terminal domain.
    • Domain
      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • Post-translational
      modifications
      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
      Sulfation of C-terminal tyrosines increases affinity for thrombin.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • FGG antibody
      • FIBG_HUMAN antibody
      • Fibrinogen gamma chain antibody
      • Fibrinogen gamma polypeptide antibody
      • fibrinogen gamma-b chain antibody
      see all

    Images

    • Anti-Fibrinogen gamma chain antibody [EPR3084] (ab92481) at 1/500 dilution + Human plasma lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 52 kDa
    • ab92481, at a 1/100 dilution, staining Fibrinogen gamma chain in paraffin embedded Human liver tissue by Immunohistochemistry.

    References

    ab92481 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your phone call. I am still waiting for information regarding the immunogens for ab92481 and ab92510, but I will let you know as soon as I have any additional information.

    DISCOUNT CODE: ***

    Expiration date: August 16t...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up