Anti-Fibrinopeptide A antibody [1F7] (ab14790)


  • Product nameAnti-Fibrinopeptide A antibody [1F7]
    See all Fibrinopeptide A primary antibodies
  • Description
    Mouse monoclonal [1F7] to Fibrinopeptide A
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein:


    , corresponding to N terminal amino acids 1-16 of Human Fibrinopeptide A (alpha chain) and conjugated to a carrier protein.

  • Positive control
    • IHC-P: FFPE human liver tissue sections.



Our Abpromise guarantee covers the use of ab14790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [1F3] to Fibrinogen (ab10066).
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibrinogen (ab34269). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Fibrinogen (ab34269) as Detection.


  • FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A alpha polypeptide antibody
    • FGA antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen alpha/alpha E chain [Precursor] antibody
    see all

Anti-Fibrinopeptide A antibody [1F7] images

  • IHC image of Fibrinopeptide A staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14790, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Standard Curve for Fibrinogen (Analyte: Fibrinogen protein (Human) (ab84410)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) at 5µg/ml and Detector Antibody Rabbit polyclonal to Fibrinogen (ab34269) at 0.5µg/ml.


References for Anti-Fibrinopeptide A antibody [1F7] (ab14790)

ab14790 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

Unfortunately we have not determined the epitope for ab14790. We only know that it reacts with fibrinopeptide A region in the alpha-chain of fibrinogen and free fibrinopeptide A.

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please find attached to this email the CoC you requested.

Vielen Dank für Ihre Anfrage. In der Tat haben wir einige Antikörper-Paare, die wir für Sandwich-ELISA (sELISA) an humanen Proben garantieren, da die verschiedenen Klone verschiedene Epitope erkennen: Der Klon [1F3] (ab10066) kann mit den Klonen ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Ewen Smith

Verified customer

Submitted Sep 09 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein
Blocking step Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Ewen Smith

Verified customer

Submitted Sep 06 2005