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ADSGEGDFLAEGGGVR, corresponding to N terminal amino acids 1-16 of Human Fibrinopeptide A (alpha chain) and conjugated to a carrier protein.
Our Abpromise guarantee covers the use of ab14790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [1F3] to Fibrinogen (ab10066).|
|WB||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibrinogen (ab34269). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Fibrinogen (ab34269) as Detection.|
IHC image of Fibrinopeptide A staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14790, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab14790 has not yet been referenced specifically in any publications.