Recombinant
RabMAb

Anti-Fibrinopeptide B antibody [EPR3082] (ab92510)

Overview

  • Product name
    Anti-Fibrinopeptide B antibody [EPR3082]
    See all Fibrinopeptide B primary antibodies
  • Description
    Rabbit monoclonal [EPR3082] to Fibrinopeptide B
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Fibrinopeptide B.

  • Positive control
    • WB: Human prostate and Human plasma lysates IHC-P: Human liver tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
  • Application notes
    ICC: 1/100.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/5000 - 1/10000. Predicted molecular weight: 56 kDa.

    Is unsuitable for IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • Involvement in disease
      Defects in FGB are a cause of thrombophilia.
      Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen.
    • Sequence similarities
      Contains 1 fibrinogen C-terminal domain.
    • Domain
      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • Post-translational
      modifications
      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epididymis secretory sperm binding protein Li 78p antibody
      • FGB antibody
      • FIBB_HUMAN antibody
      • Fibrinogen B beta polypeptide antibody
      • Fibrinogen beta chain antibody
      • Fibrinogen beta polypeptide chain antibody
      • Fibrinopeptide B antibody
      • HEL S 78p antibody
      • Liver regeneration-related protein LRRG036/LRRG043/LRRG189 antibody
      • MGC104327 antibody
      • MGC120405 antibody
      • OTTHUMP00000197066 antibody
      see all

    Images

    • All lanes : Anti-Fibrinopeptide B antibody [EPR3082] (ab92510) at 1/5000 dilution

      Lane 1 : Human prostate lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 56 kDa
    • ab92510, at a 1/50 dilution, staining Fibrinopeptide B in formalin fixed, paraffin embedded Human liver tissue by Immunohistochemistry. Detection: by DAB staining

    References

    ab92510 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your phone call. I am still waiting for information regarding the immunogens for ab92481 and ab92510, but I will let you know as soon as I have any additional information.

    DISCOUNT CODE: ***

    Expiration date: August 16t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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