Recombinant Anti-Fibrinogen beta chain antibody [EPR3083] (ab133359)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3083] to Fibrinogen beta chain
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Fibrinogen beta chain antibody [EPR3083]
See all Fibrinogen beta chain primary antibodies -
Description
Rabbit monoclonal [EPR3083] to Fibrinogen beta chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma, Human prostrate and Rat platelet lysates; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3083 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
ICC/IF
1/100 - 1/250. |
Target
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Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Involvement in disease
Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsConversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2244 Human
- Entrez Gene: 110135 Mouse
- Entrez Gene: 24366 Rat
- SwissProt: P02675 Human
- Unigene: 300774 Human
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Alternative names
- Beta fibrinogen antibody
- Epididymis Secretory Sperm Binding Protein Li 78p antibody
- FGB antibody
see all
Images
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All lanes : Anti-Fibrinogen beta chain antibody [EPR3083] (ab133359) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human prostate lysate
Lane 3 : Rat platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Actual band size : 56 kDa -
Immunofluorescence analysis of Fibrinogen beta chain in HeLa cells, using ab133359 at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133359 has been referenced in 1 publication.
- Fan SM et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 167:121-131 (2018). IHC-P ; Mouse . PubMed: 29567388