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Synthetic peptide of Human Fibroblast activation protein, alpha, corresponding to the stalk region.
(Peptide available as ab41274.)
Our Abpromise guarantee covers the use of ab28244 in the following tested applications.
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 18403594|
|Flow Cyt||1/50. PubMed: 20361950
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.|