Anti-Fibroblast activation protein, alpha antibody (ab28244)

Overview

  • Product nameAnti-Fibroblast activation protein, alpha antibody
    See all Fibroblast activation protein, alpha primary antibodies
  • Description
    Rabbit polyclonal to Fibroblast activation protein, alpha
  • SpecificityThis antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide of Human Fibroblast activation protein, alpha, corresponding to the stalk region.

    (Peptide available as ab41274.)

Properties

Applications

Our Abpromise guarantee covers the use of ab28244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 18403594
Flow Cyt 1/50. PubMed: 20361950

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.

Target

  • FunctionIn association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • Tissue specificityFibroblast specific.
  • Sequence similaritiesBelongs to the peptidase S9B family.
  • Post-translational
    modifications
    N-glycosylated.
    The N-terminus may be blocked.
  • Cellular localizationCell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt
  • Database links
  • Alternative names
    • 170 kDa melanoma membrane bound gelatinase antibody
    • 170 kDa melanoma membrane-bound gelatinase antibody
    • DPPIV antibody
    • FAP antibody
    • FAPA antibody
    • Fibroblast activation protein alpha antibody
    • Integral membrane serine protease antibody
    • SEPR_HUMAN antibody
    • Seprase antibody
    see all

Anti-Fibroblast activation protein, alpha antibody images

  • All lanes : Anti-Fibroblast activation protein, alpha antibody (ab28244) at 1/1000 dilution

    Lane 1 : Recombinant Human FAPalpha at 0.08 µg
    Lane 2 : Recombinant Human FAPalpha at 0.04 µg
    Lane 3 : Recombinant Human FAPalpha at 0.02 µg
    Lane 4 : Recombinant Human FAPalpha at 0.01 µg
    Lane 5 : Recombinant Human FAPalpha at 0.005 µg


    Predicted band size : 88 kDa
  • ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Fibroblast activation protein, alpha antibody (ab28244)

This product has been referenced in:
  • Singhal PK  et al. Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity. Proc Natl Acad Sci U S A 113:122-7 (2016). Read more (PubMed: 26699463) »
  • Li M  et al. Targeting of cancer-associated fibroblasts enhances the efficacy of cancer chemotherapy by regulating the tumor microenvironment. Mol Med Rep 13:2476-84 (2016). WB, IHC . Read more (PubMed: 26846566) »

See all 8 Publications for this product

Product Wall

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ...

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Thank you for contacting Abcam.

I would recommend using ab28244 or ab28246 as each of these is guaranteed to work in IF on rat tissues under our Abpromise.

The immunogen for ab28244 Fibroblast activation protein, alpha antibody st...

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Thank you for contacting Abcam.
We do not know of any publications which have used this product in IHC-P in mouse tissues.
A good starting point for determining the optimal concentration is to use the dilution used in the earlier referenced pa...

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Thank you for contacting us.

This product was used in IHC-P in the following publication: Bhati R et al. Molecular characterization of human breast tumor vascular cells. Am J Pathol 172:1381-90 (2008). IHC-P; Human. (PubMed: 18403594) (http:/...

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Thank you for your enquiry and your interest in our products.

The tested applications and the species are normally listed on the on-line product datasheets. Generally, we test our antibodies in human, mouse and rat samples - very few antibodi...

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Thank you for contacting Abcam regarding ab28244. Unfortunately the immunogen information for ab28244 is considered proprietary and I do not have access to that information. Please accept my apologies for not being able to provide you w...

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Thank you for your reply. I can confirm that only ab28244 (Anti-Fibroblast activation protein, alpha antibody - Stalk region) is tested and guaranteed for flow cytometry and I therefore recommend this antibody for your experiments. Please...

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Thank you for contacting us. I can confirm that ab28244 is tested and guaranteed for FACS and IHC-P in mouse, human and rat by our Abpromise. and therefore should be suitable to isolate cells that express FAP. This antibody recognizes t...

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Here is a section from a recent publication on FAP (J Clin Invest. 2009 Dec;119(12):3613-25) "FAP is a type II transmembrane cell surface protein belonging to the post-proline dipeptidyl aminopeptidase family, sharing the highest similarity with dip...

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Thank you for your enquiry. The descriptive terms for these antibodies are based on comparison with the dipeptidyl peptidase family, especially DPP-4. FAP-alpha starts with a short cytoplasmic domain followed by a transmembrane domain. The stal...

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