Anti-Fibroblast activation protein, alpha antibody (ab53066)

Overview

  • Product name
    Anti-Fibroblast activation protein, alpha antibody
    See all Fibroblast activation protein, alpha primary antibodies
  • Description
    Rabbit polyclonal to Fibroblast activation protein, alpha
  • Specificity
    ab53066 detects endogenous levels of total Fibroblast activation protein, alpha. Please note the immunogen used to produce this product has 92% homology with Mouse DPPIV/CD26.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human, Monkey
  • Immunogen

    A synthetic peptide derived from human Fibroblast activation protein, alpha. Peptide available as ab150744.

  • Positive control
    • Human breast carcinoma tissue and LOVO cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa.Can be blocked with Fibroblast activation protein or alpha peptide (ab150744).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/10000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20861157
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • Tissue specificity
    Fibroblast specific.
  • Sequence similarities
    Belongs to the peptidase S9B family.
  • Post-translational
    modifications
    N-glycosylated.
    The N-terminus may be blocked.
  • Cellular localization
    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt
  • Database links
  • Alternative names
    • 170 kDa melanoma membrane bound gelatinase antibody
    • 170 kDa melanoma membrane-bound gelatinase antibody
    • DPPIV antibody
    • FAP antibody
    • FAPA antibody
    • Fibroblast activation protein alpha antibody
    • Integral membrane serine protease antibody
    • SEPR_HUMAN antibody
    • Seprase antibody
    see all

Images

  • All lanes : Anti-Fibroblast activation protein, alpha antibody (ab53066) at 1/500 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide


    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab53066 staining Fibroblast activation protein alpha in Monkey spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with Tris/EDTA (pH9). Samples were incubated with primary antibody (1/100 in a commercial diluent) for 30 minutes at 21°C. An undiluted HRP-conjugated Goat polyclonal was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human cancer-associated fibroblasts, staining Fibroblast activation protein, alpha with ab53066.
  • ab53066 staining Fibroblast activation protein, alpha in Dog cancerous tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris/EDTA (pH 9.0) buffer. Samples were incubated with undiluted primary antibody (1/100 in antibody diluent) for 30 minutes at 21°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of murine lung fibroblasts, staining Fibroblast activation protein with ab53066.

    Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/100 in TBS) for 1 hour at 20°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Vliegen G  et al. The expression of proline-specific enzymes in the human lung. Ann Transl Med 5:130 (2017). IHC ; Human . Read more (PubMed: 28462210) »
  • Park JI  et al. Scaffold-Free Coculture Spheroids of Human Colonic Adenocarcinoma Cells and Normal Colonic Fibroblasts Promote Tumorigenicity in Nude Mice. Transl Oncol 9:79-88 (2016). IHC-P, ICC/IF ; Human . Read more (PubMed: 26947885) »

See all 36 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Fibroblast)
Permeabilization
Yes - 0.1%Triton X100
Specification
Fibroblast
Blocking step
Dako X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Username

Hongwei Shao

Verified customer

Submitted Aug 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako
Permeabilization
No
Specification
Liver
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 01 2016

We cannot say if the FAP is actually expressed in the Schwann cells without more tests. It may be upregulated in cultured cells. I see that U87 cells, a glioblastoma, were used a positive control for another FAP antibody, ab110779, but it does not foll...

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Thank you for contacting us.

You can use any of the anti FAP alpha antibody however, please check the tested species and application the antibody is tested in.

ab53066 is published in many Abreviews and publications and it is guarante...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung fibroblast)
Specification
lung fibroblast
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton x-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 04 2012

I am happy to let you know this blocking peptide has been added to our catalog as ab150744 http://www.abcam.com/fibroblast-activation-protein-alpha-peptide-ab150744.html

Thank you for contacting us.

We understand your concerns, this product has been characterized in WB so it is guaranteed for this application along with others. We are selling 10s of vials of this antibody per quarter so if it does not work wit...

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Thank you for submitting an Abreview of ab53066. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this...

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Application
Western blot
Sample
Human Tissue lysate - whole (artery)
Loading amount
10 µg
Specification
artery
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 20 2012

Can you please provide your order number for ab53066? Once I have this information I can process your request for a replacement. Are you still interested in receiving Anti-Fibroblast activation protein, alpha antibody - Catalytic insert (ab28246)? Once...

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1-10 of 26 Abreviews or Q&A

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