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Immunocytochemistry/ Immunofluorescence-Fibroblast activation protein, alpha antibody - Catalytic insert(ab28246)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Fibroblast activation protein, alpha antibody - Catalytic insert(ab28246)
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Product Name
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Fibroblast activation protein, alpha antibody - Catalytic insert
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See all Fibroblast activation protein, alpha antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Fibroblast activation protein, alpha - Catalytic insert
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Immunogen
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Synthetic peptide of Human Fibroblast activation protein, alpha, based on the insert in the catalytic domain.
(Peptide available as ab41277.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody recognizes the 759 amino acid form of Fibroblast activation protein, alpha, specifically, but does not recognize other DPP family members.
We have a range of domain specific antibodies for this target. For a full list please see all Fibroblast activation protein, alpha antibodies
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Detects a band of approximately 110-120 kDa due to post translational modifications and reduction. (Predicted molecular weight: 88 kDa). Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2 µg/ml.
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Cellular localization
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Cell Membrane; single-pass type II membrane protein. Found in cell surface lamellipodia, invadopodia and on shed vesicles.
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
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Relevance
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Fibroblast Activation Protein alpha subunit (FAPa or FAP, alpha) is a integral membrane gelatinase belonging to the serine protease family. FAPa is the alpha subunit and DPP4 (CD26) the beta subunit of a heterodimeric membrane-bound proteinase complex also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase. Some cells make only FAPa homodimers, some only DPP4 homodimers. The monomer is inactive. FAP, alpha is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.
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Alternative names Fibroblast activation protein, alpha antibody - Catalytic insert (ab28246) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Fibroblast activation protein, alpha antibody - Catalytic insert is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Fibroblast activation protein, alpha antibody - Catalytic insert images, references, products related to ab28246 and other tools.
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Fibroblast activation protein, alpha antibody - Catalytic insert images:
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 Immunocytochemistry/ Immunofluorescence-Fibroblast activation protein, alpha antibody - Catalytic insert(ab28246)
ICC/IF image of ab28246 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28246, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Fibroblast activation protein, alpha antibody - Catalytic insert(ab28246)
Ab28246 staining Human normal placenta. Staining is localised to cytoplasmic compartment.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to Fibroblast activation protein, alpha
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