Fibroblast activation protein, alpha antibody - Spacer region (ab28245)

Product Name 

Fibroblast activation protein, alpha antibody - Spacer region

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Fibroblast activation protein, alpha - Spacer region

Immunogen 

Synthetic peptide of Human Fibroblast activation protein, alpha based on the spacer region.

(Peptide available as ab41275.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody recognizes both long isoforms of Fibroblast activation protein, alpha, but does not recognize other DPP family members.

We have a range of domain specific antibodies for this target. For a full list please see all Fibroblast activation protein, alpha antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Detects a band of approximately 110-120 kDa due to post translational modifications and reduction. (Predicted molecular weight: 88 kDa). Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane; single-pass type II membrane protein. Found in cell surface lamellipodia, invadopodia and on shed vesicles.

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs

Relevance 

Fibroblast Activation Protein alpha subunit (FAPa or FAP, alpha) is a integral membrane gelatinase belonging to the serine protease family. FAPa is the alpha subunit and DPP4 (CD26) the beta subunit of a heterodimeric membrane-bound proteinase complex also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase. Some cells make only FAPa homodimers, some only DPP4 homodimers. The monomer is inactive. FAP, alpha is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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