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Fibronectin isolated from a pool of normal human plasma.
Our Abpromise guarantee covers the use of ab2413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa).|
|IP||Use at an assay dependent dilution. Used at 1ug/ml for 1 hr 30 min on renal carcinoma cell line 786-0 (see Abreview).|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution. This antibody may be diluted to a titer of 1/50 - 1/250 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.|
ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.