Full length protein, Human Fibronectin, purified from serum by affinity chromatography on gelatinsepharose.
Our Abpromise guarantee covers the use of ab80923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibronectin (ab299). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Fibronectin (ab299) as Detection.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 263 kDa.|
|IP||Use at 2 µg/mg of lysate. (Native and denatured - use Protein G)|
|Blocking||Use at an assay dependent dilution.|