Anti-Fibronectin antibody [HFN 7.1] - BSA and Azide free (ab80923)

Overview

  • Product nameAnti-Fibronectin antibody [HFN 7.1] - BSA and Azide freeSee all Fibronectin primary antibodies ...
  • Description
    Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free
  • Specificityab80923 is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface
  • Tested applicationsSandwich ELISA, WB, IP, Blocking more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, Human Fibronectin, purified from serum by affinity chromatography on gelatinsepharose.

  • EpitopePeptide core
  • Positive control
    • SW156 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab80923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibronectin (ab299). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Fibronectin (ab299) as Detection.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 263 kDa.
IP Use at 2 µg/mg of lysate. (Native and denatured - use Protein G)
Blocking Use at an assay dependent dilution.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody [HFN 7.1] - BSA and Azide free images

Protocols

References for Anti-Fibronectin antibody [HFN 7.1] - BSA and Azide free (ab80923)

ab80923 has not yet been referenced specifically in any publications.

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Reducing Bovine serum will also put the cells into a lower state of growth with reduced access to growth factors. Growth factor absence will also modulate the activation state of integrin and other cell surf...

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I found a good set of products that gives a strong sandwich ELISA signal.

Capture: ab80923

Detection: ab299

Antigen: ab81743

Click here (or use the following: http://www.abcam.com/index.html?datasheet=80923)....

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I've included a list of capture, detection, protein, and fluorescent-conjugated secondary below against Fibronectin. The capture and detection antibodies have been tested and guaranteed to work in sandwich ELISA to detect human fibronectin:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"