Overview

  • Product nameAnti-Fibulin 1 antibody
    See all Fibulin 1 primary antibodies
  • Description
    Rabbit polyclonal to Fibulin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, African Green Monkey
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 143-192 (CCVKSQETGD LDVGGLQETD KIIEVEEEQE DPYLNDRCRG GGPCKQQCRD) of Human Fibulin 1 (NP_001987).

  • Positive control
    • OVCAR-3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionIncorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
  • Tissue specificityIsoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells.
  • Involvement in diseaseNote=A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation.
    Note=Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer.
  • Sequence similaritiesBelongs to the fibulin family.
    Contains 3 anaphylatoxin-like domains.
    Contains 9 EGF-like domains.
  • Developmental stageWidely expressed during embryonic development. Prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of peripheral nerves. In embryos of gestational week (gw) 4, staining was observed in the early mesenchymal bone anlagen. In gw 6.5 and 8, all perichondrial structures showed expression but the chondrocytes themselves showed no staining. In gw 10, expression is prominent in the interterritorial matrix surrounding the hypertrophic chondrocytes.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basement membrane protein 90 antibody
    • BM 90 antibody
    • FBLN antibody
    • Fbln1 antibody
    • FBLN1_HUMAN antibody
    • FIBL 1 antibody
    • FIBL-1 antibody
    • FIBL1 antibody
    • Fibulin 1 antibody
    • Fibulin-1 antibody
    see all

Anti-Fibulin 1 antibody images

  • Anti-Fibulin 1 antibody (ab98032) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg

    Predicted band size : 77 kDa

References for Anti-Fibulin 1 antibody (ab98032)

ab98032 has not yet been referenced specifically in any publications.

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