Recombinant
RabMAb

Anti-Fibulin 1 antibody [EPR10913] (ab175204)

Overview

  • Product name
    Anti-Fibulin 1 antibody [EPR10913]
    See all Fibulin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10913] to Fibulin 1
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Fibulin 1. The exact sequence is proprietary.
    Database link: P23142

  • Positive control
    • Human placenta, fetal liver and fetal heart tissue lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab175204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 77 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
    • Tissue specificity
      Isoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells.
    • Involvement in disease
      Note=A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation.
      Note=Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer.
    • Sequence similarities
      Belongs to the fibulin family.
      Contains 3 anaphylatoxin-like domains.
      Contains 9 EGF-like domains.
    • Developmental stage
      Widely expressed during embryonic development. Prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of peripheral nerves. In embryos of gestational week (gw) 4, staining was observed in the early mesenchymal bone anlagen. In gw 6.5 and 8, all perichondrial structures showed expression but the chondrocytes themselves showed no staining. In gw 10, expression is prominent in the interterritorial matrix surrounding the hypertrophic chondrocytes.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basement membrane protein 90 antibody
      • BM 90 antibody
      • FBLN antibody
      • Fbln1 antibody
      • FBLN1_HUMAN antibody
      • FIBL 1 antibody
      • FIBL-1 antibody
      • FIBL1 antibody
      • Fibulin 1 antibody
      • Fibulin-1 antibody
      see all

    Images

    • All lanes : Anti-Fibulin 1 antibody [EPR10913] (ab175204) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Fetal liver tissue lysate
      Lane 3 : Fetal heart tissue lysate

      Lysates/proteins at 1/10 dilution per lane.


      Predicted band size : 77 kDa
      Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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