Anti-Fibulin 5 antibody [1G6A4] (ab66339)
Key features and details
- Mouse monoclonal [1G6A4] to Fibulin 5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Fibulin 5 antibody [1G6A4]
See all Fibulin 5 primary antibodies -
Description
Mouse monoclonal [1G6A4] to Fibulin 5 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant His tagged fragment, corresponding to amino acids 242-448 of Human Fibulin 5
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Positive control
- Colon and breast cancer
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR171104-21 are from Tissue Culture Supernatant
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1G6A4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
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IHC-P |
1/500 - 1/2000.
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
IHC-P
1/500 - 1/2000. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling. -
Tissue specificity
Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes. -
Involvement in disease
Defects in FBLN5 are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. Autosomal dominant cutis laxa is a relatively benign inherited and acquired connective tissue disorder.
Defects in FBLN5 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected.
Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3) [MIM:608895]. ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the fibulin family.
Contains 6 EGF-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10516 Human
- Omim: 604580 Human
- SwissProt: Q9UBX5 Human
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Alternative names
- ADCL2 antibody
- ARCL1A antibody
- ARMD3 antibody
see all
Images
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Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/2000 dilution + Human vascular smooth muscle whole cell lysate at 20 µg
Secondary
HRP-conjugated Rabbit anti-mouse IgG polyclonal at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/500 dilution + Immunogen (recombinant truncated protein) at 1 µg
Predicted band size: 50 kDa -
Immunohistochemical analysis of paraffin embedded human colon cancer tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
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Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
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ICC/IF image of ab66339 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66339, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab66339 has been referenced in 16 publications.
- Yan Y et al. Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells. Exp Ther Med 22:896 (2021). PubMed: 34257709
- Chakraborty M et al. Primary Human Trabecular Meshwork Model for Pseudoexfoliation. Cells 10:N/A (2021). PubMed: 34943956
- Li R et al. FBLN5 is targeted by microRNA-27a-3p and suppresses tumorigenesis and progression in high-grade serous ovarian carcinoma. Oncol Rep 44:2143-2151 (2020). PubMed: 32901854
- O'Neill Moore S et al. Insights into the biophysical forces between proteins involved in elastic fiber assembly. J Mater Chem B 8:9239-9250 (2020). PubMed: 32966543
- Chen Y et al. Fibulin-5 contributes to colorectal cancer cell apoptosis via the ROS/MAPK and Akt signal pathways by downregulating transient receptor potential cation channel subfamily V member 1. J Cell Biochem 120:17838-17846 (2019). PubMed: 31148262