The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/850. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionAn adhesion molecule that interacts with extracellular matrix molecules in developing teeth and may play important roles in differentiation and maintenance of odontoblasts as well as in dentin formation.
Sequence similaritiesBelongs to the fibulin family. Contains 2 EGF-like domains. Contains 1 Sushi (CCP/SCR) domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.