Overview

  • Product name
  • Description
    Rabbit polyclonal to Fibulin 7
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ASQNCLSKQQ LLSAIRQLQQ LLKGQETRFA EGIRHMKSRL AALQNSVGRV GPDALPVSCP ALNTPADGRK FGSKYLVDHE, corresponding to amino acids 23-102 of Human Fibulin 7 (UniProt ID: Q53RD9).

  • Positive control
    • Human colon tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/850. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    An adhesion molecule that interacts with extracellular matrix molecules in developing teeth and may play important roles in differentiation and maintenance of odontoblasts as well as in dentin formation.
  • Sequence similarities
    Belongs to the fibulin family.
    Contains 2 EGF-like domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • FBLN7 antibody
    • FBLN7_HUMAN antibody
    • FIBL-7 antibody
    • Fibl7 antibody
    • Fibulin-7 antibody
    • TM14 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cell junctions. Recommended concentration of ab122240 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122240, at a dilution of 1/850, staining Fibulin 7 in paraffin embedded Human colon tissue by Immunohistochemistry.

References

ab122240 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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