Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab173725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 24 kDa.

Target

  • Function
    Germline specific transcription factor implicated in postnatal oocyte-specific gene expression. Plays a key regulatory role in the expression of multiple oocyte-specific genes, including those that initiate folliculogenesis and those that encode the zona pellucida (ZP1, ZP2 and ZP3) required for fertilization and early embryonic survival. Essential for oocytes to survive and form primordial follicles. The persistence of FIGLA in adult females suggests that it may regulate additional pathways that are essential for normal ovarian development. Binds to the E-box (5'-CANNTG-3') of the ZPs (ZP1, ZP2, ZP3) promoters.
  • Tissue specificity
    Germ cells. Expressed in the fetal ovary, but not by a range of other tissues. Expression increases across mid-gestation, rising some 40-fold by the time of primordial follicle formation.
  • Involvement in disease
    Defects in FIGLA are the cause of premature ovarian failure type 6 (POF6) [MIM:612310]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities
    Contains 1 bHLH (basic helix-loop-helix) domain.
  • Developmental stage
    Expressed in ovarian follicles (from the primordial through to the secondary stage), in mature oocytes, and less frequently in preimplantation embryos.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc8 antibody
    • Class C basic helix-loop-helix protein 8 antibody
    • Factor in the germline alpha antibody
    • FIGalpha antibody
    • FIGLA antibody
    • FIGLA_HUMAN antibody
    • Folliculogenesis specific basic helix loop helix antibody
    • Folliculogenesis-specific basic helix-loop-helix protein antibody
    • POF6 antibody
    • Transcription factor FIGa antibody
    see all

Images

  • Anti-FIGLA antibody (ab173725) at 1/100 dilution + T47D cell line lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 24 kDa
  • IHC-P image of FIGLA staining with ab173725 on tissue sections from adult marmoset ovary. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab173725 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

References

ab173725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (Adult ovary)
Specification
Adult ovary
Permeabilization
No
Fixative
Formaldehyde
Username

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Verified customer

Submitted Aug 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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