Germline specific transcription factor implicated in postnatal oocyte-specific gene expression. Plays a key regulatory role in the expression of multiple oocyte-specific genes, including those that initiate folliculogenesis and those that encode the zona pellucida (ZP1, ZP2 and ZP3) required for fertilization and early embryonic survival. Essential for oocytes to survive and form primordial follicles. The persistence of FIGLA in adult females suggests that it may regulate additional pathways that are essential for normal ovarian development. Binds to the E-box (5'-CANNTG-3') of the ZPs (ZP1, ZP2, ZP3) promoters.
Germ cells. Expressed in the fetal ovary, but not by a range of other tissues. Expression increases across mid-gestation, rising some 40-fold by the time of primordial follicle formation.
Involvement in disease
Defects in FIGLA are the cause of premature ovarian failure type 6 (POF6) [MIM:612310]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Contains 1 bHLH (basic helix-loop-helix) domain.
Expressed in ovarian follicles (from the primordial through to the secondary stage), in mature oocytes, and less frequently in preimplantation embryos.
Folliculogenesis specific basic helix loop helix antibody
Folliculogenesis-specific basic helix-loop-helix protein antibody
Transcription factor FIGa antibody
Western blot - Anti-FIGLA antibody (ab173725)
Anti-FIGLA antibody (ab173725) at 1/100 dilution + T47D cell line lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIGLA antibody (ab173725)This image is courtesy of an abreview from Zachary Yu-Ching Lin
IHC-P image of FIGLA staining with ab173725 on tissue sections from adult marmoset ovary. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab173725 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.