Overview

  • Product nameAnti-FIGNL1 antibody
    See all FIGNL1 primary antibodies
  • Description
    Rabbit polyclonal to FIGNL1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human FIGNL1 (NP_071399.2)

  • Positive control
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab70841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

Anti-FIGNL1 antibody images

  • All lanes : Anti-FIGNL1 antibody (ab70841) at 1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of Human FIGNL1 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab70841 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using 1 µg/mg ab70841 and chemiluminescence with an exposure time of 30 seconds.

References for Anti-FIGNL1 antibody (ab70841)

ab70841 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"