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Rabbit polyclonal to FIGNL1
Predicted to work with:
Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human FIGNL1 (NP_071399.2)
Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa).
Use at 2-5 µg/mg of lysate.
May regulate osteoblast proliferation and differentiation.
Fidgetin like protein 1 antibody
Western blot - FIGNL1 antibody (ab70841)
All lanes :
Anti-FIGNL1 antibody (ab70841) at 1 µg/ml
Lane 1 :
Whole cell lysate from HeLa cells at 50 µg
Lane 2 :
Whole cell lysate from HeLa cells at 15 µg
Lane 3 :
Whole cell lysate from HeLa cells at 5 µg
Lane 4 :
Whole cell lysate from 293T cells at 50 µg
Lane 5 :
Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique
Predicted band size :
Observed band size :
71 kDa (
why is the actual band size different from the predicted?
Exposure time :
Immunoprecipitation - FIGNL1 antibody (ab70841)
Detection of Human FIGNL1 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab70841 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using 1 µg/mg ab70841 and chemiluminescence with an exposure time of 30
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"