• Product name
  • Description
    Rabbit polyclonal to Filaggrin
  • Specificity
    ab81468 reacts specifically with the human 435 kDa protein.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the N terminal domain of human Filaggrin

  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4. Lot numbers other than GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.



Our Abpromise guarantee covers the use of ab81468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/1000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 435 kDa.
ICC/IF Use at an assay dependent concentration.


  • Function
    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • Tissue specificity
    Keratohyalin granules.
  • Involvement in disease
    Ichthyosis vulgaris
    Dermatitis atopic 2
  • Sequence similarities
    Belongs to the S100-fused protein family.
    Contains 2 EF-hand domains.
    Contains 23 filaggrin repeats.
  • Post-translational
    Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
    Undergoes deimination of some arginine residues (citrullination).
  • Information by UniProt
  • Database links
  • Alternative names
    • ATOD2 antibody
    • Epidermal filaggrin antibody
    • FILA_HUMAN antibody
    • Filaggrin antibody
    • Filaggrin precursor antibody
    • Fillagrin antibody
    • FLG antibody
    • Profilaggrin antibody
    see all


  • Immunohistochemical analysis of Human skin sample labeling filaggrin with ab81468. Counterstained with haematoxylin. 

  • ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Shin JM  et al. Targeted deletion of Crif1 in mouse epidermis impairs skin homeostasis and hair morphogenesis. Sci Rep 7:44828 (2017). IHC-P . Read more (PubMed: 28317864) »
  • Lin Z  et al. Loss-of-function mutations in CAST cause peeling skin, leukonychia, acral punctate keratoses, cheilitis, and knuckle pads. Am J Hum Genet 96:440-7 (2015). Read more (PubMed: 25683118) »

See all 4 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EnVision™ FLEX Target Retrieval Solution, High pH (50x). Tris/EDTA buffer, pH 9 de Dako
Blocking step
EnVision™ FLEX Peroxidase-Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 4% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jan 17 2017

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Vielen Dank für Ihre Rückmeldung. Aus unseren Unterlagen geht hervor, dass der Antikörper im Western blot routinemäßig in einer Verdünnungsreihe getestet wurde, die wie auf dem Datenblatt angegeben Verdünnungen zwischen 1:500 und 1:5000 beinhaltete....

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Thank you for your email. I am very sorry that it took so long to get back to you. I have been in contact with the lab that produces this antibody and it was very difficult to get satisfactory information about ab81468. According to the informati...

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