Overview

  • Product nameAnti-Filaggrin antibody [FLG01]
    See all Filaggrin primary antibodies
  • Description
    Mouse monoclonal [FLG01] to Filaggrin
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein.

  • Positive control
    • Skin.
  • General notesMolecular weight of Filaggrin = 38 kDa (Filaggrin) and 40 kDa (Pro Filaggrin).

Properties

Applications

Our Abpromise guarantee covers the use of ab3137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Target

  • FunctionAggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • Tissue specificityKeratohyalin granules.
  • Involvement in diseaseIchthyosis vulgaris
    Dermatitis atopic 2
  • Sequence similaritiesBelongs to the S100-fused protein family.
    Contains 2 EF-hand domains.
    Contains 23 filaggrin repeats.
  • Post-translational
    modifications
    Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
    Undergoes deimination of some arginine residues (citrullination).
  • Information by UniProt
  • Database links
  • Alternative names
    • Epidermal filaggrin antibody
    • FILA_HUMAN antibody
    • Filaggrin antibody
    • Filaggrin precursor antibody
    • Fillagrin antibody
    • FLG antibody
    • Profilaggrin antibody
    see all

Anti-Filaggrin antibody [FLG01] images

  • Overlay histogram showing A431 cells stained with ab3137 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3137, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab3137 staining Filaggrin in Human skin tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde, blocked with 1% BSA for 30 minutes at 25°C and permeabilized with 0.2% Triton X-100. Samples were incubated with primary antibody (1/200 in diluent) for 1 hour. An AlexaFluor®594-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of cultured Human keratinocytes isolated from neonatal foreskin, staining Filaggrin with ab3137. Antigen retrieval was performed via heat mediation in citrate buffer, blocked in 3% BSA for 20 min, before incubating with primary antibody overnight at 4°C.

References for Anti-Filaggrin antibody [FLG01] (ab3137)

This product has been referenced in:
  • Chen Y  et al. Highly Rapid and Efficient Conversion of Human Fibroblasts to Keratinocyte-Like Cells. J Invest Dermatol N/A:N/A (2013). Read more (PubMed: 23921950) »
  • Benavente CA  et al. Effects of Niacin Restriction on Sirtuin and PARP Responses to Photodamage in Human Skin. PLoS One 7:e42276 (2012). IHC-P ; Human . Read more (PubMed: 22860104) »

See all 3 Publications for this product

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I am afraid he epitope of ab3137 has not been determined.

However, Abcam offers a 6 months guarantee for all of our products (Abpromise). In the event that a product is not functioning in the application...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Trititon X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

Submitted Jul 13 2012

Thank you for contacting Abcam.

The antibody ab3137 does recognize Filiaggrin and pro-filligrin, however we have not tested this antibody in IF, therefore you may be eligible for our testing discount program, see the link below:

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Thank you for your enquiry. To our knowledge this antibody has yet to be tested in species other than those specified on our product datasheet. This doesn't mean that it will not work on this species. But, we cannot guarantee that this antibody will wo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"