Overview

  • Product nameAnti-Filamin A antibody
    See all Filamin A primary antibodies
  • Description
    Goat polyclonal to Filamin A
  • SpecificityAntiserum specifically stains filamin associated with stress fibers and membrane ruffles by immunofluorescent labeling of monolayer cultured cells. The product reacts best in cultured chicken fibroblasts. Good immunofluorescent labeling may also be obtained with cells of other species. In immunoblotting, antiserum specifically stains the filamin band.
  • Tested applicationsSuitable for: IP, ICC/IF, WB, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Chicken, Human, Xenopus laevis
    Predicted to work with: Zebrafish
  • Immunogen

    Full length native protein (purified) (Chicken).

  • General notesStorage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.


    Filamin is a large flexible actin cross-linking protein present in vertebrates in both muscle and non-muscle cells. Filamin has a polypeptide molecular weight of approximately 250,000 daltons and forms dimers in solution. Filamin has been primarily purified from smooth muscle but was also identified in macrophages, neutrophils, platelets, and other cell types. Filamin cross-links actin and promotes its aggregation and gelation. Immunofluorescent labeling of a large variety of cells with Goat Anti-Filamin reveals an extensive association of the protein with the actin containing stress fibers. Goat Anti-Filamin may be used for immunofluorescent localization of filamin in cultured cells and tissues, and to study the state of actin organization in muscle cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
  • PurityWhole antiserum
  • Primary antibody notesFilamin is a large flexible actin cross-linking protein present in vertebrates in both muscle and non-muscle cells. Filamin has a polypeptide molecular weight of approximately 250,000 daltons and forms dimers in solution. Filamin has been primarily purified from smooth muscle but was also identified in macrophages, neutrophils, platelets, and other cell types. Filamin cross-links actin and promotes its aggregation and gelation. Immunofluorescent labeling of a large variety of cells with Goat Anti-Filamin reveals an extensive association of the protein with the actin containing stress fibers. Goat Anti-Filamin may be used for immunofluorescent localization of filamin in cultured cells and tissues, and to study the state of actin organization in muscle cells.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. (PMID 18495106).
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 250 kDa.
ICC Use at an assay dependent concentration.
Flow Cyt 1/250. (see Abreview).



ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPromotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in FLNA are the cause of periventricular nodular heterotopia type 1 (PVNH1) [MIM:300049]; also called nodular heterotopia, bilateral periventricular (NHBP or BPNH). PVNH is a developmental disorder characterized by the presence of periventricular nodules of cerebral gray matter, resulting from a failure of neurons to migrate normally from the lateral ventricular proliferative zone, where they are formed, to the cerebral cortex. PVNH1 is an X-linked dominant form. Heterozygous females have normal intelligence but suffer from seizures and various manifestations outside the central nervous system, especially related to the vascular system. Hemizygous affected males die in the prenatal or perinatal period.
    Defects in FLNA are the cause of periventricular nodular heterotopia type 4 (PVNH4) [MIM:300537]; also known as periventricular heterotopia Ehlers-Danlos variant. PVNH4 is characterized by nodular brain heterotopia, joint hypermobility and development of aortic dilation in early adulthood.
    Defects in FLNA are the cause of otopalatodigital syndrome type 1 (OPD1) [MIM:311300]. OPD1 is an X-linked dominant multiple congenital anomalies disease mainly characterized by a generalized skeletal dysplasia, mild mental retardation, hearing loss, cleft palate, and typical facial anomalies. OPD1 belongs to a group of X-linked skeletal dysplasias known as oto-palato-digital syndrome spectrum disorders that also include OPD2, Melnick-Needles syndrome (MNS), and frontometaphyseal dysplasia (FMD). Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. FLNA is a widely expressed protein that regulates re-organization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes and second messengers. Males with OPD1 have cleft palate, malformations of the ossicles causing deafness and milder bone and limb defects than those associated with OPD2. Obligate female carriers of mutations causing both OPD1 and OPD2 have variable (often milder) expression of a similar phenotypic spectrum.
    Defects in FLNA are the cause of otopalatodigital syndrome type 2 (OPD2) [MIM:304120]; also known as cranioorodigital syndrome. OPD2 is a congenital bone disorder that is characterized by abnormally modeled, bowed bones, small or absent first digits and, more variably, cleft palate, posterior fossa brain anomalies, omphalocele and cardiac defects.
    Defects in FLNA are the cause of frontometaphyseal dysplasia (FMD) [MIM:305620]. FMD is a congenital bone disease characterized by supraorbital hyperostosis, deafness and digital anomalies.
    Defects in FLNA are the cause of Melnick-Needles syndrome (MNS) [MIM:309350]. MNS is a severe congenital bone disorder characterized by typical facies (exophthalmos, full cheeks, micrognathia and malalignment of teeth), flaring of the metaphyses of long bones, s-like curvature of bones of legs, irregular constrictions in the ribs, and sclerosis of base of skull.
    Defects in FLNA are the cause of X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX) [MIM:300048]. CIIPX is characterized by a severe abnormality of gastrointestinal motility due to primary qualitative defects of enteric ganglia and nerve fibers. Affected individuals manifest recurrent signs of intestinal obstruction in the absence of any mechanical lesion.
    Defects in FLNA are the cause of FG syndrome type 2 (FGS2) [MIM:300321]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
    Defects in FLNA are the cause of terminal osseous dysplasia (TOD) [MIM:300244]. A rare X-linked dominant male-lethal disease characterized by skeletal dysplasia of the limbs, pigmentary defects of the skin and recurrent digital fibroma during infancy. A significant phenotypic variability is observed in affected females.
    Defects in FLNA are the cause of cardiac valvular dysplasia X-linked (CVDX) [MIM:314400]. A rare X-linked heart disease characterized by mitral and/or aortic valve regurgitation. The histologic features include fragmentation of collagenous bundles within the valve fibrosa and accumulation of proteoglycans, which produces excessive valve tissue leading to billowing of the valve leaflets.
  • Sequence similaritiesBelongs to the filamin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 24 filamin repeats.
  • DomainComprised of a NH2-terminal actin-binding domain, 24 internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation extent changes in response to cell activation.
    The N-terminus is blocked.
  • Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABP 280 antibody
    • ABP-280 antibody
    • Actin-binding protein 280 antibody
    • Alpha filamin antibody
    • Alpha-filamin antibody
    • APBX antibody
    • CSBS antibody
    • CVD1 antibody
    • Endothelial actin binding protein antibody
    • Endothelial actin-binding protein antibody
    • Filamin 1 antibody
    • Filamin A alpha antibody
    • Filamin A antibody
    • Filamin-1 antibody
    • Filamin-A antibody
    • FLN antibody
    • FLN-A antibody
    • FLN1 antibody
    • FLNA antibody
    • FLNA_HUMAN antibody
    • FMD antibody
    • MNS antibody
    • NHBP antibody
    • Non muscle filamin antibody
    • Non-muscle filamin antibody
    • OPD antibody
    • OPD1 antibody
    • OPD2 antibody
    • XLVD antibody
    • XMVD antibody
    see all

Anti-Filamin A antibody images

  • ab11074 staining Filamin A in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated donkey anti-goat IgG (H+L) at a 1/500 dilution.

    US: Unstained, Red Peak;
    IgG Ms: IgG Mouse (Isotype Control), Blue Peak;
    Filamin: Filamin A, Green peak.

    See Abreview

  • ab11074 at a 1/2000 dilution staining Filamin A in XTC embryonic polyclonal fibroblasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with formaldehyde, permeabilized using Triton X-100 and blocked with 1% BSA. The secondary used was a Texas Red conjugated anti-goat, 1/200 dilution.
  • Anti-Filamin A antibody (ab11074) at 1/6000 dilution + Human platelet lysate at 20 µg

    Secondary
    Rabbit anti-Goat HRP at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 250 kDa
    Observed band size : >250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Mahesh Shivananjappa, United States

    See Abreview

References for Anti-Filamin A antibody (ab11074)

This product has been referenced in:
  • Samwer M  et al. The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis. EMBO J 32:1886-902 (2013). Xenopus laevis . Read more (PubMed: 23727888) »
  • Castoria G  et al. Androgen-induced cell migration: role of androgen receptor/filamin a association. PLoS One 6:e17218 (2011). ICC/IF ; Mouse . Read more (PubMed: 21359179) »

See all 4 Publications for this product

Product Wall

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Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 24 2012

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. Unfortunately, ab11074 has not been tested in IHC and we do not actually know i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20% Criterion 26 Well)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Nov 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (0.5 embryo was loaded or 10 to the 4 XTC cells)
Loading amount 0.5 cells
Specification 0.5 embryo was loaded or 10 to the 4 XTC cells
Gel Running Conditions Reduced Denaturing (5-15% gradient SDS page)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Helene Cousin

Verified customer

Submitted Jul 23 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (XTC cell-embryonic polyclonal fibroblast)
Specification XTC cell-embryonic polyclonal fibroblast
Fixative Formaldehyde
Permeabilization Yes - 0.5% TritonX100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. Helene Cousin

Verified customer

Submitted Jul 22 2008

Thank you for your enquiry. The source used to create this antibody was chicken gizzard. This antibody is non-specific. It was not created against a specific sub-type, so it should work well on all sub-types. This is all the information I can provide y...

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