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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Filamin A (phospho S2152) + Filamin B (phospho S2107) aa 2100-2200.
Database link: P21333
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab75978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|ELISA||Use at an assay dependent concentration. PubMed: 21507248|
|WB||1/10000 - 1/20000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunofluorescence analysis of U. parvum infected BPH-1 (Human benign prostate) cells staining Filamin A (phospho S2152) and Filamin B (phospho S2107) with ab75978 (red). Cells were fixed with formaldehyde before incubating with primary antibody. An AlexaFluor®594-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ab75978 at 1/100 dilution staining Filamin A (phospho S2152) and Filamin B (phospho S2107) in human uterus by Immunohistochemistry, Paraffin-embedded tissue.
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