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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Filamin A (phospho S2152) + Filamin B (phospho S2107) aa 2100-2200.
Database link: P21333
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab75978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|ELISA||Use at an assay dependent concentration. PubMed: 21507248|
|WB||1/10000 - 1/20000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunofluorescence analysis of U. parvum infected BPH-1 (Human benign prostate) cells staining Filamin A (phospho S2152) and Filamin B (phospho S2107) with ab75978 (red). Cells were fixed with formaldehyde before incubating with primary antibody. An AlexaFluor®594-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ab75978 at 1/100 dilution staining Filamin A (phospho S2152) and Filamin B (phospho S2107) in human uterus by Immunohistochemistry, Paraffin-embedded tissue.