Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978)

Overview

  • Product nameAnti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY]
  • Description
    Rabbit monoclonal [EP2310AY] to Filamin A (phospho S2152) + Filamin B (phospho S2107)
  • Specificityab75978 detects Filamin A phosphorylated on serine 2152 and Filamin B phosphorylated on serine 2107.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Filamin A (phospho S2152) + Filamin B (phospho S2107) aa 2100-2200.
    Database link: P21333

  • Positive control
    • HeLa cell lysates, untreated or treated with calyculin A; human uterus tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab75978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
ELISA Use at an assay dependent concentration. PubMed: 21507248
WB 1/10000 - 1/20000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • RelevanceFilamin A-P21333. Function: Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Filamin B-O75369. Function: Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
    • Database links
    • Alternative names
      • ABP-278 antibody
      • ABP-280 antibody
      • ABP-280 homolog antibody
      • Actin-binding protein 280 antibody
      • Actin-binding-like protein antibody
      • Alpha-filamin antibody
      • Beta-filamin antibody
      • Endothelial actin-binding protein antibody
      • Fh1 antibody
      • Filamin homolog 1 antibody
      • Filamin-1 antibody
      • Filamin-3 antibody
      • Filamin-A antibody
      • Filamin-B antibody
      • FLN antibody
      • FLN-A antibody
      • FLN1 antibody
      • FLN1L antibody
      • FLN3 antibody
      • FLNA antibody
      • FLNB antibody
      • Non-muscle filamin antibody
      • TABP antibody
      • TAP antibody
      • Thyroid autoantigen antibody
      • Truncated ABP antibody
      • Truncated actin-binding protein antibody
      see all

    Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] images

    • All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/20000 dilution

      Lane 1 : HeLa lysate, untreated
      Lane 2 : HeLa lysate, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 281 kDa
      Observed band size : 281 kDa
    • Immunofluorescence analysis of U. parvum infected BPH-1 (Human benign prostate) cells staining Filamin A (phospho S2152) and Filamin B (phospho S2107) with ab75978 (red). Cells were fixed with formaldehyde before incubating with primary antibody. An AlexaFluor®594-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    • ab75978 at 1/100 dilution staining Filamin A (phospho S2152) and Filamin B (phospho S2107) in human uterus by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978)

    This product has been referenced in:
    • Allam AB  et al. Ureaplasma parvum infection alters filamin A dynamics in host cells. BMC Infect Dis 11:101 (2011). ICC/IF, ELISA ; Rat . Read more (PubMed: 21507248) »
    • Bogatcheva NV  et al. Molecular mechanisms mediating protective effect of cAMP on lipopolysaccharide (LPS)-induced human lung microvascular endothelial cells (HLMVEC) hyperpermeability. J Cell Physiol 221:750-9 (2009). WB ; Human . Read more (PubMed: 19725051) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your recent telephone enquiry and for your patience. It has taken some time to obtain the immunogen information for you.

    For the two rabbit monoclonal antibodies:

    ab76289
    Immunogen: from within amino acids range...

    Read More

    Thank you for your inquiry. HeLa cells are serum-starved overnight, and then treated with 100 nM Calyculin A for 30 min at 37 deg C. Calyculin A was chosen because it is a known serine/threonine phosphatase inhibitor. 1.Resjö, S. et al. ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"