• Product nameAnti-Filamin antibody [FLMN01]
  • Description
    Mouse monoclonal [FLMN01] to Filamin
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Human
  • Immunogen

    Tissue, cells or virus corresponding to Filamin. Tissue/ cell preparation - platelet filamin

  • Epitopenot determined
  • Positive control
    • WB: NIH3T3 cells or mouse cells-2 lysate. IHC: Skin IF/ICC: A431 cell line.



Our Abpromise guarantee covers the use of ab80837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 281 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.


Anti-Filamin antibody [FLMN01] images

  • ICC/IF image of ab80837 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80837, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Filamin antibody [FLMN01] (ab80837) at 2 µg/ml + Mouse cells-2 lysate

    Predicted band size : 281 kDa
    This image is produced using the version of this antibody, containing BSA and azide.
  • Formalin-fixed, paraffin-embedded human skin stained with ab80837, at 1 - 2 µg/ml, using a peroxidase-conjugated secondary antibody and AEC chromogen. Note cytoplasmic staining of smooth muscle cells surrounding blood vessels.

    This image was produced using the version of this antibody, containing BSA and azide.
  • Overlay histogram showing A431 cells stained with ab80837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80837, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Filamin antibody [FLMN01] (ab80837)

This product has been referenced in:

See 1 Publication for this product

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For the two rabbit monoclonal antibodies:

Immunogen: from within amino acids range...

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