Overview

  • Product name
    Anti-Filamin antibody [FLMN01]
  • Description
    Mouse monoclonal [FLMN01] to Filamin
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Human
  • Immunogen

    Tissue, cells or virus corresponding to Filamin. Tissue/ cell preparation - platelet filamin

  • Epitope
    not determined
  • Positive control
    • WB: NIH3T3 cells or mouse cells-2 lysate. IHC: Skin IF/ICC: A431 cell line.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab80837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 281 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-Filamin antibody [FLMN01] images

  • ICC/IF image of ab80837 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80837, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Filamin antibody [FLMN01] (ab80837) at 2 µg/ml + Mouse cells-2 lysate

    Predicted band size : 281 kDa
    This image is produced using the version of this antibody, containing BSA and azide.
  • Formalin-fixed, paraffin-embedded human skin stained with ab80837, at 1 - 2 µg/ml, using a peroxidase-conjugated secondary antibody and AEC chromogen. Note cytoplasmic staining of smooth muscle cells surrounding blood vessels.

    This image was produced using the version of this antibody, containing BSA and azide.
  • Overlay histogram showing A431 cells stained with ab80837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80837, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Filamin antibody [FLMN01] (ab80837)

This product has been referenced in:
  • Li N  et al. Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis. FASEB J 29:3788-805 (2015). Read more (PubMed: 26048141) »
  • Wan HT  et al. Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study. Endocrinology 155:249-62 (2014). Read more (PubMed: 24169556) »

See all 3 Publications for this product

Product Wall

Thank you for your recent telephone enquiry and for your patience. It has taken some time to obtain the immunogen information for you.

For the two rabbit monoclonal antibodies:

ab76289
Immunogen: from within amino acids range...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (testis and seminoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0, 20min, 100C
Permeabilization
No
Specification
testis and seminoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up