Overview

  • Product nameAnti-Filamin B antibody
    See all Filamin B primary antibodies
  • Description
    Rabbit polyclonal to Filamin B
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to Human Filamin B.
    Database link: O75369

  • Positive control
    • Human controls: Colon Carcinoma, Prostate Carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionConnects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
    • Tissue specificityUbiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.
    • Involvement in diseaseNote=Interaction with FLNA may compensate for dysfunctional FLNA homodimer in the periventricular nodular heterotopia (PVNH) disorder.
      Defects in FLNB are the cause of atelosteogenesis type 1 (AO1) [MIM:108720]; also known as giant cell chondrodysplasia or spondylohumerofemoral hypoplasia. Atelosteogenesis are lethal short-limb skeletal dysplasias with vertebral abnormalities, disharmonious skeletal maturation, poorly modeled long bones and joint dislocations.
      Defects in FLNB are the cause of atelosteogenesis type 3 (AO3) [MIM:108721]. Atelosteogenesis are short-limb lethal skeletal dysplasias with vertebral abnormalities, disharmonious skeletal maturation, poorly modeled long bones and joint dislocations. In AO3 recurrent respiratory insufficiency and/or infections usually result in early death.
      Defects in FLNB are the cause of boomerang dysplasia (BOOMD) [MIM:112310]. This is a perinatal lethal osteochondrodysplasia characterized by absence or underossification of the limb bones and vertebre. Boomerang dysplasia is distinguished from atelosteogenesis on the basis of a more severe defect in mineralisation, with complete absence of ossification in some limb elements and vertebral segments.
      Defects in FLNB are the cause of Larsen syndrome (LRS) [MIM:150250]. An osteochondrodysplasia characterized by large-joint dislocations and characteristic craniofacial abnormalities. The cardinal features of the condition are dislocations of the hip, knee and elbow joints, with equinovarus or equinovalgus foot deformities. Spatula-shaped fingers, most marked in the thumb, are also present. Craniofacial anomalies include hypertelorism, prominence of the forehead, a depressed nasal bridge, and a flattened midface. Cleft palate and short stature are often associated features. Spinal anomalies include scoliosis and cervical kyphosis. Hearing loss is a well-recognized complication.
      Defects in FLNB are the cause of spondylocarpotarsal synostosis syndrome (SCT) [MIM:272460]; also known as spondylocarpotarsal syndrome (SCT) or congenital synspondylism or vertebral fusion with carpal coalition or congenital scoliosis with unilateral unsegmented bar. The disorder is characterized by short stature and vertebral, carpal and tarsal fusions.
    • Sequence similaritiesBelongs to the filamin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 24 filamin repeats.
    • DomainComprised of a NH2-terminal actin-binding domain, 24 internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation. The first hinge region prevents binding to ITGA and ITGB subunits.
    • Post-translational
      modifications
      ISGylation prevents ability to interact with the upstream activators of the JNK cascade and inhibits IFNA-induced JNK signaling.
    • Cellular localizationCytoplasm > cytoskeleton. Polarized at the periphery of myotubes; Cytoplasm > cytoskeleton. Predominantly localized at actin stress fibers and Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. In differentiating myotubes, isoform 1, isoform 2 and isoform 3 are localized diffusely throughout the cytoplasm with regions of enrichment at the longitudinal actin stress fiber. In differentiated tubes, isoform 1 is also detected within the Z-lines.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABP 278 antibody
      • ABP 280 homolog antibody
      • ABP-278 antibody
      • ABP-280 homolog antibody
      • ABP278 antibody
      • Actin binding like protein antibody
      • Actin binding protein 278 antibody
      • Actin-binding-like protein antibody
      • AOI antibody
      • Beta filamin antibody
      • Beta-filamin antibody
      • DKFZp686A1668 antibody
      • DKFZp686O033 antibody
      • Fh1 antibody
      • Filamin 1 actin binding protein 280 like antibody
      • Filamin 3 antibody
      • Filamin B antibody
      • Filamin B beta actin binding protein 278 antibody
      • Filamin homolog 1 antibody
      • Filamin-3 antibody
      • Filamin-B antibody
      • FLN B antibody
      • FLN-B antibody
      • FLN1L antibody
      • FLN3 antibody
      • FLNB antibody
      • FLNB_HUMAN antibody
      • LRS1 antibody
      • SCT antibody
      • TABP antibody
      • TAP antibody
      • Thyroid autoantigen antibody
      • Truncated ABP antibody
      • Truncated actin binding protein antibody
      • Truncated actin-binding protein antibody
      see all

    Anti-Filamin B antibody images

    • ab84905, at a 1/500 dilution, staining Filamin B in formalin fixed, paraffin embedded human prostate carcinoma tissue by Immunohistochemistry. Detection: red fluorescent, highly cross absorbed goat anti-rabbit IgG antibody used at a dilution of 1/100.

    • ab84905, at a 1/500 dilution, staining Filamin B in formalin fixed, paraffin embedded human prostate carcinoma tissue by Immunohistochemistry, using DAB staining.

    References for Anti-Filamin B antibody (ab84905)

    ab84905 has not yet been referenced specifically in any publications.

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