Mouse, Human Predicted to work with:
Synthetic peptide corresponding to Filamin C. The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Filamin C that contains serine 2146.
C2C12, HeLa, HEK293 + EGF.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free) as a carrier. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Filamin C. The final product is generated by affinity chromatography using a Filamin C-derived peptide that is phosphorylated at serine 2113.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 291 kDa.
RelevanceFLNC is a muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z-disks in muscle cells.
Defects in FLNC are the cause of autosomal dominant filaminopathy. Myofibrillar myopathy (MFM) is a neuromuscular disorder, usually with an adult onset, characterized by focal myofibrillar destruction and pathological cytoplasmic protein aggregations. Autosomal dominant filaminopathy is a form of MFM characterized by morphological features of MFM and clinical features of a limb-girdle myopathy. A heterozygous nonsense mutation which segregates with the disease, has been identified in the FLNC gene.
Western blot - Anti-Filamin C (phospho S2113) antibody (ab20485)
All lanes : Anti-Filamin C (phospho S2113) antibody (ab20485) at 1/1000 dilution
Lane 1 : HeLa cell lysate + Lambda phosphatase Lane 2 : HeLa cell lysate Lane 3 : HeLa cell lysate with a non-phosphorylated peptide corresponding to the immunogen Lane 4 : HeLa cell lysate with a generic phosphoserine-containing peptide Lane 5 : HeLa cell lysate with a phosphopeptide corresponding to Filamin C (phospho S2113) Lane 6 : Mouse myoblast cell lysate
Predicted band size : 291 kDa
Right panel: Lysates from HEK293 cells untreated (left lanes) or treated with EGF (right lanes). Western blotting was performed using the Filamin C (phospho S2113) antibody (upper blot) or an antibody recognizing total filamin protein (lower blot).
References for Anti-Filamin C (phospho S2113) antibody (ab20485)
has not yet been referenced specifically in any publications.
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