The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Involvement in diseaseDefects in BFSP1 are the cause of cataract cortical juvenile-onset (CCJO) [MIM:611391]. A juvenile-onset cataract with opacities restricted to the cortex of the lens, not involving the nucleus.
Sequence similaritiesBelongs to the intermediate filament family.
Cellular localizationMembrane. Cytoplasm. Cytoplasm > cytoskeleton. Membrane- and cytoskeleton-associated.