The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged Filensin is approximately 30ng/ml if used as a capture antibody.
1/500 - 1/1000. Detects a band of approximately 85 kDa.
Involvement in diseaseDefects in BFSP1 are the cause of cataract cortical juvenile-onset (CCJO) [MIM:611391]. A juvenile-onset cataract with opacities restricted to the cortex of the lens, not involving the nucleus.
Sequence similaritiesBelongs to the intermediate filament family.
Cellular localizationMembrane. Cytoplasm. Cytoplasm > cytoskeleton. Membrane- and cytoskeleton-associated.