Anti-FIP200 antibody (ab21852)

Overview

  • Product nameAnti-FIP200 antibody
    See all FIP200 primary antibodies
  • Description
    Mouse polyclonal to FIP200
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Fusion protein corresponding to Fruit fly (Drosophila melanogaster) FIP200 aa 215-314.
    Sequence:

    DFHGFDELLDNDDVFTAQQQPTPQQQALTESSQADSPNKKLKMGDEVETE SGEQTERGVQTLTSSSSLARRPNLNLLQWISSKGNHRALQLMSDECIQGL

  • General notes

    Produced from outbred CD1 mice.

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab21852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 38 kDa.

Target

  • FunctionImplicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. Required for autophagosome formation (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways.
  • Tissue specificityExpression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
  • Developmental stageExpression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay.
  • Cellular localizationNucleus. Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Alternative names
    • FAK family kinase-interacting protein of 200 kDa antibody
    • FIP200 antibody
    • RB1-inducible coiled-coil protein 1 antibody
    • RB1CC1 antibody
    • RBCC1_HUMAN antibody
    see all

Anti-FIP200 antibody images

  • All lanes : Anti-FIP200 antibody (ab21852) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 38 kDa
    The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.

References for Anti-FIP200 antibody (ab21852)

ab21852 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (extracts prepared from whole Drosophila larvae)
Loading amount 30 µg
Specification extracts prepared from whole Drosophila larvae
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 09 2012

I am very pleased to hear you would like to accept our offer and test ab21852 in endogenous protein. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forendogenous proteinand incl...

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Thank you very much for your interest in ab21852.

You are correct that, since we have only tested ab21852 for reactivity to the recombinant immunogen we can't guarantee that it will react with endogenous protein. However, if you are interest...

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Thank you for contacting us. Because we carry over 77,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"