Anti-Firefly Luciferase antibody (ab21176)
- Datasheet
- Specific References (14)
- Protocols
Overview
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Product nameAnti-Firefly Luciferase antibody
See all Firefly Luciferase primary antibodies -
DescriptionRabbit polyclonal to Firefly Luciferase
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SpecificityThe antibody identifies by immunocyotochemistry recombinant luciferase in cultured HEK293 cells transfected with a plasmid bearing the luciferase gene.
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Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
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Species reactivityReacts with: Firefly
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Immunogen
Full length native protein (purified) (Firefly (Photinus pyralis)).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 -
Concentration information loading... -
PurityIgG fraction
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab21176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-Fr | 1/1000. PubMed: 18219389 | |
| WB | 1/1000 - 1/2000. |
Target
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RelevanceLuciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
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Cellular localizationPeroxisome
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Database links
- SwissProt: P08659 Firefly
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Alternative names
- ec 1.13.12.7 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
Anti-Firefly Luciferase antibody images
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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)ab21176 at 10 - 20 µg/mL staining Luciferase in transfected HEK293 cells by ICC/IF. The cells were fixed with methanol and acetone. A FITC conjugated Anti-Rabbit IgG was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - firefly Luciferase antibody (ab21176)This image is courtesy of an Abreview submitted by Dr Mal Niladriab21176 at 1/200 dilution staining engineered adult rat stromal stem cells by ICC/IF. The cells were paraformaldehyde (2%) fixed and Triton X-100 (0.1%) was used for cell permealization (15 min incubation time). The cells were incubated with the antibody overnight at 4C. The image shows Luciferase (green-upper right panel), counterstained cell nuclei (DAPI-blue-upper left panel), overlay (lower left panel) and a phase contrast image (lower right panel). The image was taken with a confocal laser scanning microscope equipped with an additional laser differential Interference Contrast (DIC) mode.
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Western blot - Anti-Firefly Luciferase antibody (ab21176)All lanes : Anti-Firefly Luciferase antibody (ab21176) at 1/1000 dilution
Lane 1 : Lysates of HEK-293T cells overexpressing luciferase
Lane 2 : Lysates of HEK-293T cells overexpressing luciferase with Luciferase Immunizing Peptide
Secondary
Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate -
Immunohistochemistry (Frozen sections) - Anti-Firefly Luciferase antibody (ab21176)This image is courtesy of an Abreview submitted by Radhi Praba Velayuthamab21176 staining Firefly Luciferase in Mouse cerebellum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with PBS + 0.1% Triton X-100 (PBST) for 10 minutes and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/1500 in 10% Goat serum in PBST) for 1 hour at 22°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Firefly Luciferase antibody (ab21176)This image is courtesy of an anonymous Abreviewab21176 staining mouse mammary carcinoma cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking with a commercial blocking agent. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa Fluor® 555 conjugated goat anti-rabbit antibody was used as the secondary. The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)Immunocytochemical analysis analysis of HEK-293T cells overexpressing luciferase labelling Luciferase with ab21176 at a concentration of 10μg/mL. The secondary antibody was a Goat Anti-Rabbit IgG, FITC conjugate.
Protocols
References for Anti-Firefly Luciferase antibody (ab21176)
This product has been referenced in:
- Parkins KM et al. A multimodality imaging model to track viable breast cancer cells from single arrest to metastasis in the mouse brain. Sci Rep 6:35889 (2016). IHC ; Mouse . Read more (PubMed: 27767185) »
- Hayashi M et al. Whole-Body In Vivo Monitoring of Inflammatory Diseases Exploiting Human Interleukin 6-Luciferase Transgenic Mice. Mol Cell Biol 35:3590-601 (2015). IHC . Read more (PubMed: 26283726) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"