Overview

  • Product nameAnti-Firefly Luciferase antibody (HRP)
    See all Firefly Luciferase primary antibodies
  • Description
    Goat polyclonal to Firefly Luciferase (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: Dot Blot, ELISA, WBmore details
  • Species reactivity
    Reacts with: Firefly (Photinus pyralis)
  • Immunogen

    Luciferase [Photinus pyralis (Firefly)].

  • Positive control
    • Assayed against 1.0µg of firefly luciferase in a standard capture ELISA.
  • General notes

    Conjugated to horseradish peroxidase (HRP). Conjugation reference: Farr & Nakane, J.Immunol.Methods 47; 129-144. (1981)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent dilution.
ELISA 1/50000 - 1/100000.
WB 1/10000. See Abreview.

Target

  • RelevanceLuciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • Cellular localizationPeroxisome
  • Database links
    • Alternative names
      • ec 1.13.12.7 antibody
      • Firefly antibody
      • Luciferase antibody
      • Luciferin 4 monooxygenase antibody

    References for Anti-Firefly Luciferase antibody (HRP) (ab635)

    This product has been referenced in:
    • MacKay JA  et al. HIV TAT peptide modifies the distribution of DNA nanolipoparticles following convection-enhanced delivery. Mol Ther 16:893-900 (2008). Read more (PubMed: 18388927) »
    • Nasim MT & Eperon IC A double-reporter splicing assay for determining splicing efficiency in mammalian cells. Nat Protoc 1:1022-8 (2006). Goat . Read more (PubMed: 17406339) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry. We only have the product sizes available as they are noted on our online datasheets. We typically do not offer small sample sizes. We do guarantee our antibodies based on the tested applications and species reactivities. For...

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    Thank you very much for your enquiry. Unfortunately, I have not been able to obtain any positive data for either formalin or frozen samples from our laboratory. As such, I feel that we should not be marketing this for use in IHC or IM and I have...

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    I'm sorry to hear you are experiencing problems with ab635. We have had very few complaints about ab635 and none with the lot you are using. I understand this is a replacement vial after you experienced problems with ab3279 and after trying my proto...

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    Further to our telephone conversation this morning I have been in correspondence with the source of this antibody. They confirmed with me that this antibody is yet to be tested using IHC-Fr. They could not tell me where the information regarding the di...

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    Thank you for sending us your detailed protocol. We are very sorry to hear that you are having problem with this antibody. We would like to draw your attention to the fact that this antibody has only been tested and characterized for IHC on frozen ...

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    No, this antibody has not been tested in a Sandwich ELISA, nor have any of our other antibodies to fire fly luciferase. However, we recommend that you use the mouse monoclonal ab7358 on the bottoem (i.e. as a coating antibody)and a conjugated version o...

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    ab635 has not been tested for this purpose. A potential problem is that some fixation techniques destroy the epitopes to which the antibodies bind. I believe that this technique has been shown to work with a similar antibody but I do not have the refer...

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    This antibody can be used for direct detection of luciferose in Western blots using any suitable HRP substrate e.g. ECL.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"