Anti-FITC antibody [2A3] (ab10257)

Overview

  • Product name
    Anti-FITC antibody [2A3]
    See all FITC primary antibodies
  • Description
    Mouse monoclonal [2A3] to FITC
  • Specificity
    ab10257 reacts with both free and conjugated FITC.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Immunogen

    FITC-BSA conjugate.

  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purity tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    2A3
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

References

ab10257 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. We have no vials of GR4859-5 but we do have six vials of GR4859-6, which is the same stock as GR4859-5, but aliquotted on a different date. Please do not hesitate to contact us if you need any more advice or ...

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Unfortunately ab10257 has not been tested to react with FAM. I am sorry for not being more helpful on this occasion.

Thank you for your enquiry. Ab10257 can be used in IHC in two ways: 1. You can use FITC-labeled antibody against antigen of your interest as first antibody and then apply peroxidase-labeled ab10257 as developing reagent. 2. You can add the exces...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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