Anti-FITC antibody [7F4] (ab116449)
Key features and details
- Mouse monoclonal [7F4] to FITC
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-FITC antibody [7F4]
See all FITC primary antibodies -
Description
Mouse monoclonal [7F4] to FITC -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Conjugate of Bovine serum albumin (BSA) and Fluorescein isothiocyanate (FITC).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. -
Storage buffer
pH: 7.40
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity is > 95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
7F4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab116449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 1 kDa.
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ELISA |
Use at an assay dependent dilution.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 1 kDa. |
ELISA
Use at an assay dependent dilution. |
Target
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Relevance
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. -
Alternative names
- Fluorescein Isothiocyanate antibody
Datasheets and documents
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Datasheet download
References (0)
ab116449 has not yet been referenced specifically in any publications.