Overview

  • Product nameAnti-FITC antibody [7F4]
    See all FITC primary antibodies
  • Description
    Mouse monoclonal [7F4] to FITC
  • Specificityab116449 reacts with FITC, PCR products labelled with FITC, and proteins labelled with FITC.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Conjugate of Bovine serum albumin (BSA) and Fluorescein isothiocyanate (FITC).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurity is > 95% by SDS-PAGE.
  • ClonalityMonoclonal
  • Clone number7F4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 1 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

References for Anti-FITC antibody [7F4] (ab116449)

ab116449 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"