• FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab19224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100.
ICC 1/200 - 1/2000.
WB Use at an assay dependent dilution. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000.
IP Use at an assay dependent dilution. PubMed: 17052454


  • RelevanceFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

References for Anti-FITC antibody (ab19224)

This product has been referenced in:
  • Zhang M  et al. Expression levels of seprase/FAPa and DPPIV/CD26 in epithelial ovarian carcinoma. Oncol Lett 10:34-42 (2015). Human . Read more (PubMed: 26170973) »
  • Takada K  et al. Targeted disruption of the BCL9/ß-catenin complex inhibits oncogenic Wnt signaling. Sci Transl Med 4:148ra117 (2012). WB . Read more (PubMed: 22914623) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

The antibodies are from different animals and are manufactured in different laboratories so we can expect some variation. The product ab19224 is against FITC which is a derivative of Flouroscein.

I hope th...

Read More

Thank you very much for your interest in ab19224. To our knowledge, ab19224 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab19224 now, test it inIHC-Fr and submit feedback to us in the form of an Abre...

Read More

Thank you for your enquiry. Ab19224 reacts specifically with FITC and there is no reaction with KLH. The antibody has been characterized for application in Western blot, ELISA, and Immunocytochemistry. We don't have any images available at this time, b...

Read More

Thank you for your enquiry. It appears there was an error on the datasheet. This antibody has not been tested in IHC. I have updated the datasheet to reflect this, and I apologize for any inconvenience this may have caused. I hope this informat...

Read More