Anti-FITC antibody (Alkaline Phosphatase) (ab49368)

Overview

  • Product nameAnti-FITC antibody (Alkaline Phosphatase)
    See all FITC primary antibodies
  • Description
    Rabbit polyclonal to FITC (Alkaline Phosphatase)
  • ConjugationAlkaline Phosphatase
  • SpecificityThe conjugate may be used for detection of FITC labeled moieties and for visualization of immunofluorescence by light microscopy.
  • Tested applicationsSuitable for: Indirect ELISA, IHC-P, Dot blotmore details
  • Immunogen

    FITC (fluorescein isothiocyanate) conjugated to BSA

  • General notes

    The purified IgG fraction of antiserum is adsorbed to remove any antibodies to BSA and then conjugated to alkaline phosphatase.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8.00
    Preservative: 0.097% Sodium azide
    Constituents: 50% Glycerol, 1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/30000.
IHC-P 1/100.
Dot blot 1/300000.

Target

  • RelevanceFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

References for Anti-FITC antibody (Alkaline Phosphatase) (ab49368)

ab49368 has not yet been referenced specifically in any publications.

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We definitely haven't tested whether this antibody would cross-react with 6-FAM. All we can really say is that, as the immunogen used to raise the ...

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