Anti-FITC antibody (Alkaline Phosphatase) (ab49368)

Overview

  • Product name
    Anti-FITC antibody (Alkaline Phosphatase)
    See all FITC primary antibodies
  • Description
    Rabbit polyclonal to FITC (Alkaline Phosphatase)
  • Conjugation
    Alkaline Phosphatase
  • Specificity
    The conjugate may be used for detection of FITC labeled moieties and for visualization of immunofluorescence by light microscopy.
  • Tested applications
    Suitable for: Indirect ELISA, IHC-P, Dot blotmore details
  • Immunogen

    FITC (fluorescein isothiocyanate) conjugated to BSA

  • General notes

    The purified IgG fraction of antiserum is adsorbed to remove any antibodies to BSA and then conjugated to alkaline phosphatase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.097% Sodium azide
    Constituents: 50% Glycerol, 1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/30000.
IHC-P 1/100.
Dot blot 1/300000.

Target

  • Relevance
    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

References

ab49368 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

Ihave received the following information from the lab:

We definitely haven't tested whether this antibody would cross-react with 6-FAM. All we can really say is that, as the immunogen used to raise the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up