Overview

  • Product name
    Anti-FITC antibody [F4/1]
    See all FITC primary antibodies
  • Description
    Mouse monoclonal [F4/1] to FITC
  • Tested applications
    Suitable for: IP, IHC-Fr, Flow Cytmore details
  • Immunogen

    FITC conjugated to KLH

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    F4/1
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. acetone-fixed
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

Anti-FITC antibody [F4/1] images

  • Fluorescence quenching of FITC-labelled CD45 antibody using ab112511 at 10 µg/ml. Control IgG1

  • Fluorescence quenching of FITC-labelled B220 antibody using ab112511 at 10 µg/ml. Control IgG1

  • Fluorescence quenching of FITC-labelled CD8 antibody using ab112511 at 10 µg/ml. Control IgG1

References for Anti-FITC antibody [F4/1] (ab112511)

This product has been referenced in:
  • Poeter M  et al. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63. Nat Commun 5:3738 (2014). Read more (PubMed: 24769558) »
  • Balogh P  et al. Hapten-mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody. J Immunol Methods 169:35-40 (1994). Read more (PubMed: 7510754) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details. I am sorry to hear that these antibodies did not work in ICC/IF.
As requested, I have issued a free of charge replacement of ab3851, ab52060, and ab112511. These will be shipped out tonight for delivery tomor...

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Thank you for contacting Abcam.

I have been in contact with the lab and while we have not tested this clone directly with acetylated FITC, the antibody was tested with carboxy-fluorescein diacetate succinimydil ester (CFSE) in immunohistochem...

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Thank you for contacting Abcam. I am replying to your question on behalf of my colleague Ada. The antibodies that have FITC conjugated to them do not need the anti-FITC antibody ab112511, if you simply want to detect FITC fluorescence by flow cytometry...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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