Anti-FITC antibody (Phycoerythrin) (ab25539)

Overview

  • Product nameAnti-FITC antibody (Phycoerythrin)
    See all FITC primary antibodies
  • Description
    Sheep polyclonal to FITC (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityAb25539 recognises FITC.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, Dot blotmore details
  • Immunogen

    Chemical/ Small Molecule: Fluorescein Isothiocyanate (Isomer I).

  • General notes


    Ab25539 shows cross absorption with mouse, human and rat serum proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAb25539 is purified by affinity chromatography.
  • Primary antibody notesAb25539 shows cross absorption with mouse, human and rat serum proteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC/IF Use µg for 10 cells.
Dot blot Use at an assay dependent dilution.

Target

  • RelevanceFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • Alternative names
    • Fluorescein Isothiocyanate antibody

References for Anti-FITC antibody (Phycoerythrin) (ab25539)

ab25539 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Yes, this antibody would be suitable for that purpose and has been tested for ICC/IF.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us and for presenting your questions so clearly! To amplify the the signal from the FITC-conjugated anti-Legionella antibody, your idea of using an Alexa Fluor 488-conjugated anti-FITC is a good one. We unfortunately do not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"