Overview

  • Product name
    FITC Fast Conjugation Kit
    See all FITC kits
  • Product overview

    The Abcam FITC Fast Conjugation Kit (ab188285) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules with fluorescein (FITC) with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

    Fluorescein (FITC) is a fluorophore widely used in biological analysis, with a pH dependent absorption and emission spectrum. In water it shows an absorption maximum at 494 nm and an emission maximum at 520 nm.

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (1 - 2 mg scale)

    30 μg containing 3 reactions (each for 10 - 20 μg)

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab188285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

Protocols

References

ab188285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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