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The Abcam FITC Fast Conjugation Kit (ab188285) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules with fluorescein (FITC) with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
Fluorescein (FITC) is a fluorophore widely used in biological analysis, with a pH dependent absorption and emission spectrum. In water it shows an absorption maximum at 494 nm and an emission maximum at 520 nm.
The kit comes in 3 sizes for labelling recommended amounts of antibody:
300 μg containing 3 reactions (each for 100 - 200 μg)
1 mg containing 1 reaction (1 - 2 mg scale)
30 μg containing 3 reactions (each for 10 - 20 μg)
The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.
|Components||300 µg||1 mg||30 µg|
|Fluorescein Conjugate||3 vials||1 vial||3 vials|
|Fluorescein Modifier reagent||1 vial||1 vial||1 vial|
|Fluorescein Quencher reagent||1 vial||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab188285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
ab188285 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"