Anti-FIV p24 antibody [PAK3-2C1] (Biotin) (ab53265)

Overview

  • Product nameAnti-FIV p24 antibody [PAK3-2C1] (Biotin)
    See all FIV p24 primary antibodies
  • Description
    Mouse monoclonal [PAK3-2C1] to FIV p24 (Biotin)
  • ConjugationBiotin
  • SpecificityRecognises feline immunodeficiency virus (FIV) p24 gag. Does not recognise Feline Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus.
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Feline Immunodeficiency Virus.
  • Immunogen

    Virus producer molecular clone of FIV.

  • EpitopeThe specific binding epitope recognised by clone PAK3-2C1 is an immunodominant p24 epitope and it appears to be enclosed in a tight peptide coil during gag production and maturation.
  • General notesab53265 recognises a different epitope to ab54075.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone numberPAK3-2C1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesELISA: Use at an assay dependent dilution.

    Note: The addition of 0.3% Triton-X detergent is recommended to reveal this
    epitope for antibody binding.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. p24 (Capsid protein) - part of the gag polyprotein, forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
    • Alternative names
      • Capsid protein p24 antibody
      • Feline immunodeficiency virus antibody
      • Feline immunodeficiency virus capsid protein p24 antibody
      • FIV capsid protein p24 antibody

    References for Anti-FIV p24 antibody [PAK3-2C1] (Biotin) (ab53265)

    ab53265 has not yet been referenced specifically in any publications.

    Product Wall

    Indeed I can confirm that the ab54075 (clone NM3-6A9) recognizes a different epitope to ab53265 (clone PAK3-2C1) and that therefore they should be able to be used together in a Sandwich ELISA. I can also confirm that the biotinylated ab53265 is best if...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"