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Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) | Cytopainter (ab176745) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation. The approximate fluorescence excitation is 649 nm and emission maximium is 660 nm. The Excitation source is 633 nm.
The dye is designed to label cells at Ex/ Em = 649/660 nm. The Excitation source is 633 nm.
|DMSO||1 x 200µl|
|Tracking dye Deep Red||1 vial|
Our Abpromise guarantee covers the use of ab176745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
Jurkat cells were treated and stained with Tracking Dye Deep Red. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 630 nm / 660 nm (FL4). The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells
Fluorescent imaging of HeLa cells fixed with formaldehyde and labeled with ab176745 in a black wall/ clear bottom 96 well plate
ab176745 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"