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Fixable Cell Viability Assay Kit (Fluorometric - Green) | Cytopainter (ab176742) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation. The approximate fluorescence excitation is 498 nm and emission maximium is 521 nm. The Excitation source is 488 nm.
|DMSO||1 x 200µl|
|Tracking dye Green||1 vial|
Our Abpromise guarantee covers the use of ab176742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
Fluorescent imaging of HeLa cells using ab176742. HeLa cells were treated and stained with Tracking Dye Green. The cells were fixed in 3.7% formaldehyde and analyzed by fluorescence microscopy.
Detection of Jurkat cell viability using Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green) (ab176742). Jurkat cells were treated and stained with Tracking Dye Green. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 488 nm /520 nm (FL1). The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.
ab176742 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"