Anti-FK506 antibody [FK1] (ab8316)

Overview

  • Product nameAnti-FK506 antibody [FK1]
  • Description
    Mouse monoclonal [FK1] to FK506
  • SpecificityRecognizes conjugated and non-conjugated FK-506 with apparent dissociation constant Kd=10-9M.
  • Tested applicationsSuitable for: ELISAmore details
  • General notes


    FK506 antigen is a novel macrolide with similar but more potent immunosuppressant activity than cyclosporine. FK506 is used for primary or rescue immunosuppression in patients after solid organ transplantation. Clinically relevant FK506 side effects are nephrotoxicity, neurotoxicity, diabetes mellitus and hypertension. Most of these side effects are more closely related to FK 506 blood levels than dosage; therefore therapeutic drug monitoring (TDM) of FK506 levels is a prerequisite for therapy. Whole blood is the matrix of choice for FK506 TDM. The whole blood target level range aimed for in clinical practice is between 3.0 and 20.0 ng/ml and depends on the patient’s liver function, the type of organ transplanted and the time after transplantation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityPurified IgM
  • Purification notesPurified by euglobulin precipitation and dialysis. Purity is tested by electrophoresis.
  • Primary antibody notesFK506 antigen is a novel macrolide with similar but more potent immunosuppressant activity than cyclosporine. FK506 is used for primary or rescue immunosuppression in patients after solid organ transplantation. Clinically relevant FK506 side effects are nephrotoxicity, neurotoxicity, diabetes mellitus and hypertension. Most of these side effects are more closely related to FK 506 blood levels than dosage; therefore therapeutic drug monitoring (TDM) of FK506 levels is a prerequisite for therapy. Whole blood is the matrix of choice for FK506 TDM. The whole blood target level range aimed for in clinical practice is between 3.0 and 20.0 ng/ml and depends on the patient’s liver function, the type of organ transplanted and the time after transplantation.
  • ClonalityMonoclonal
  • Clone numberFK1
  • MyelomaSp2/0
  • IsotypeIgM
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • Alternative names
    • Fujimycin antibody
    • Tacrolimus antibody

References for Anti-FK506 antibody [FK1] (ab8316)

ab8316 has not yet been referenced specifically in any publications.

Product Wall

Leider wurde das Epitop dieses Antikörpers nicht weiter bestimmt, so dass ich Ihnen in diesem Fall nicht weiterhelfen kann.

Thank you for your enquiry. Regarding which FK506 position(s) were used to attach it to the carrier protein during the immunogen preparation: Succinic anhydride was used to modify the hydroxy-groups. I hope this information helps, please do not...

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Thank you for your enquiry. Quantitative determination of FK-506 is possible only by using a competitive method. We have used the antibodies in the following way: 1. FK-506-BSA was coated on a plate. 2. Mab FK1 and sample were added simulta...

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There's the problem - you have to use an -anti-mouse IgM secondary antibody as the isotype is IgM and not IgG. If you look at the online datasheet for ab8316, there is a list of compatible secondaries that can be used with ab8316.

I'm sorry to hear that you are experiencing trouble with this antibody. Can I confirm - the secondary that you are using, is it goat-anti-mouse IgM? Regarding the positive control, the originator of ab8316 has informed me that they did not test the ant...

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Thank you for your enquiry and for your patience. At this time all the information that we have regarding this antibody is located on the online datasheet. The originator of ab8316 has informed me that they did not test the antibody in Western blotting...

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Thank you for your enquiry. We currently do not carry the antigen/chemical. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free sear...

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Yes, FK506 is a immunosupressive drug recognised by this mouse monoclonal antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"