Overview

  • Product nameFK506 (Tacrolimus)
  • Description
    Immunosuppressant
  • Alternative names
    • Tacrolimus
  • Biological descriptionImmunosuppressant. Complexes with FKBP-12 to inhibit calcineurin.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120223 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    FK506 (Tacrolimus) images

    • ab32518 staining IkBα/β in RAW 264.7 cells treated with FK506 (ab120223), by ICC/IF. Decrease in IkBα/&beta expression correlates with increased concentration of FK506, as described in literature.
      The cells were incubated at 37°C for 3h in media containing different concentrations of ab120223 (FK506) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32518 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for FK506 (Tacrolimus) (ab120223)

    This product has been referenced in:
    • Kay JE Structure-function relationships in the FK506-binding protein (FKBP) family of peptidylprolyl cis-trans isomerases. Biochem J 314 ( Pt 2):361-85 (1996). Read more (PubMed: 8670043) »
    • Cardenas ME  et al. Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin. Curr Opin Nephrol Hypertens 4:472-7 (1995). Read more (PubMed: 8591053) »
    • Liu J FK506 and ciclosporin: molecular probes for studying intracellular signal transduction. Trends Pharmacol Sci 14:182-8 (1993). Read more (PubMed: 7692652) »

    See all 2 Publications for this product

    Product Wall

    J’ai regardé dans la littérature et FK506 n’est malheureusement pas soluble dans l’eau.
    FK506 est soluble dans des solvants organiques par exemple : Méthanol, Éthanol, Acétone, Éthyle Ac&e...

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    Thank you for your enquiry and interest in our products.
    We tested to see if this product could be dissolved in saline, however it could not be solubilised.
    It is known that FK506 (Tacrolimus) is soluble to around 100 mM in ethanol.
    I wo...

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    Thank you for contacting us.
    1. Has ab120223 been used in in-vivo experiments in general and for the rejection of the implant especially.
    We have not tested in our laboratory but the same chemical has been published used for in-vivo studies. <...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"