The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Belongs to the FKBP-type PPIase family. FKBP1 subfamily. Contains 1 PPIase FKBP-type domain.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg) Lane 3: SH-SY5Y whole cell lysate (20 µg) Lane 4: Human brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab24373 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab24373 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP12 knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab24373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-FKBP12 antibody (ab24373)
All lanes : Anti-FKBP12 antibody (ab24373) at 1 µg/ml
Lane 1 : Human Brain Tissue Lysate Lane 2 : Mouse Brain Tissue Lysate Lane 3 : Rat Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa Observed band size : 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FKBP12 antibody (ab24373)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ab24373 staining FKBP12 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde before heat mediated antigen retrieval and then blocked for 45 minutes at 20°C (5 minutes of peroxidase block and 10 minutes of protein block). The primary antibody was diluted, 1/2000 in PBS and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.