Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).

Target

  • Function
    May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities
    Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12 kDa FK506-binding protein antibody
    • 12 kDa FKBP antibody
    • Calstabin 1 antibody
    • FK506 binding protein 1 antibody
    • FK506 binding protein 12 antibody
    • FK506 binding protein 1A (12kD) antibody
    • FK506 binding protein 1A 12kDa antibody
    • FK506 binding protein 1A antibody
    • FK506 binding protein T cell 12 kD antibody
    • FK506 binding protein, T cell, 12 kD antibody
    • FK506 binding protein12 antibody
    • FK506-binding protein 1A antibody
    • FKB1A_HUMAN antibody
    • FKBP 12 antibody
    • FKBP 1A antibody
    • FKBP-12 antibody
    • FKBP-1A antibody
    • FKBP1 antibody
    • FKBP12 antibody
    • FKBP12 Exip3 antibody
    • FKBP12C antibody
    • fkbp1a antibody
    • Immunophilin FKBP12 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
    • PKC12 antibody
    • PKCI2 antibody
    • PPIase antibody
    • PPIase FKBP1A antibody
    • Protein kinase C inhibitor 2 antibody
    • Rotamase antibody
    see all

Images

  • All lanes : Anti-FKBP12 antibody (ab24373) at 1 µg/ml

    Lane 1 : Human Brain Tissue Lysate
    Lane 2 : Mouse Brain Tissue Lysate
    Lane 3 : Rat Brain Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • ab24373 staining FKBP12 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde before heat mediated antigen retrieval and then blocked for 45 minutes at 20°C (5 minutes of peroxidase block and 10 minutes of protein block). The primary antibody was diluted, 1/2000 in PBS and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

References

This product has been referenced in:
  • Hofhuis J  et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). Read more (PubMed: 28104817) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antibody Diluent (Dako)
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Cerebellum)
Loading amount
30 µg
Specification
Cerebellum
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. Gwen Lagoda

Verified customer

Submitted Jul 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Major Pelvic Ganglia)
Loading amount
30 µg
Specification
Major Pelvic Ganglia
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. Gwen Lagoda

Verified customer

Submitted Jun 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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